Construct: ORF TRCN0000488954
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019442.1_s317c1
- DNA Barcode:
- AGTTGGGTTATTAATCTCGCTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- F2RL1 (2150)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488954
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2150 | F2RL1 | F2R like trypsin receptor 1 | NM_005242.6 | 99.7% | 99.4% | 89G>A;621T>C;1191_1192insG |
| 2 | human | 2150 | F2RL1 | F2R like trypsin receptor 1 | XM_017009223.1 | 93% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1269
- ORF length:
- 1194
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgcgg agccccagcg cggcgtggct gctgggggcc gccatcctgc 121 tagcagcctc tctctcctgc agtggcacca tccaaggaac caatagatcc tctaaaggaa 181 gaagccttat tggtaaggtt gatggcacat cccacgtcac tggaaaagga gttacagttg 241 aaacagtctt ttctgtggat gagttttctg catctgtcct cactggaaaa ctgaccactg 301 tcttccttcc aattgtctac acaattgtgt ttgtggtggg tttgccaagt aacggcatgg 361 ccctgtgggt ctttcttttc cgaactaaga agaagcaccc tgctgtgatt tacatggcca 421 atctggcctt ggctgacctc ctctctgtca tctggttccc cttgaagatt gcctatcaca 481 tacatggcaa caactggatt tatggggaag ctctttgtaa tgtgcttatt ggctttttct 541 atggcaacat gtactgttcc attctcttca tgacctgcct cagtgtgcag aggtattggg 601 tcatcgtgaa ccccatgggg cactccagga agaaggcaaa cattgccatt ggcatctccc 661 tggcaatatg gctgctgatt ctgctggtca ccatcccttt gtatgtcgtg aagcagacca 721 tcttcattcc tgccctgaac atcacgacct gtcatgatgt tttgcctgag cagctcttgg 781 tgggagacat gttcaattac ttcctctctc tggccattgg ggtctttctg ttcccagcct 841 tcctcacagc ctctgcctat gtgctgatga tcagaatgct gcgatcttct gccatggatg 901 aaaactcaga gaagaaaagg aagagggcca tcaaactcat tgtcactgtc ctggccatgt 961 accTGATCTG CTTCACTCCT AGTAACCTTC TGCTTGTGGT GCATTATTTT CTGATTAAGA 1021 GCCAGGGCCA GAGCCATGTC TATGCCCTGT ACATTGTAGC CCTCTGCCTC TCTACCCTTA 1081 ACAGCTGCAT CGACCCCTTT GTCTATTACT TTGTTTCACA TGATTTCAGG GATCATGCAA 1141 AGAACGCTCT CCTTTGCCGA AGTGTCCGCA CTGTAAAGCA GATGCAAGTA TCCCTCACCT 1201 CAAAGAAACA CTCCAGGAAA TCCAGCTCTT ACTCTTCAAG TTCAACCACT GTTAAGACCT 1261 CCTATGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1321 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1381 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGTTGGGTT ATTAATCTCG CTTTCACGCG 1441 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt