Construct: ORF TRCN0000488964
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021593.1_s317c1
- DNA Barcode:
- TCCTCCACCGTCACTCCTGTCCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- UHMK1 (127933)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488964
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127933 | UHMK1 | U2AF homology motif kinase 1 | NM_144624.2 | 99.9% | 99.7% | 770T>C |
| 2 | human | 127933 | UHMK1 | U2AF homology motif kinase 1 | NM_175866.5 | 81.8% | 81.1% | (many diffs) |
| 3 | human | 127933 | UHMK1 | U2AF homology motif kinase 1 | NM_001184763.1 | 62.7% | 60.3% | (many diffs) |
| 4 | mouse | 16589 | Uhmk1 | U2AF homology motif (UHM) k... | XM_006496682.3 | 92.6% | 98.5% | (many diffs) |
| 5 | mouse | 16589 | Uhmk1 | U2AF homology motif (UHM) k... | NM_010633.3 | 75.9% | 80.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1101
- ORF length:
- 1032
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcgggatcc ggctgcgcct ggggcgcgga gccgccgcgt tttctggagg 121 ccttcgggcg gctgtggcag gtacagagcc gtctgggtag cggctcctcc gcctcggtgt 181 atcgggttcg ctgctgcggc aaccctggct cgccccccgg cgccctcaag cagttcttgc 241 cgccaggaac caccggggct gcggcctctg ccgccgagta tggtttccgc aaagagaggg 301 cggcgctgga acagttgcag ggtcacagaa acatcgtgac tttgtatgga gtgtttacaa 361 tccacttttc tccaaatgtg ccatcacgct gtctgttgct tgaactcctg gatgtcagtg 421 tttcggaatt gctcttatat tccagtcacc agggttgttc catgtggatg atacagcatt 481 gtgcccgaga tgttttggag gcccttgctt ttcttcatca tgagggctat gtccatgcgg 541 acctcaaacc acgtaacata ttgtggagtg cagagaatga atgttttaaa ctcattgact 601 ttggacttag cttcaaagaa ggcaatcagg atgtaaagta tattcagaca gacgggtatc 661 gggctccaga agcagaattg caaaattgct tggcccaggc tggcctgcag agtgatacag 721 aatgtacctc agctgttgat ctgtggagcc taggaatcat tttactGGAA ATGTTCTCAG 781 GAATGAAACT GAAACATACA GTCAGATCTC AGGAATGGAA GGCAAACAGT TCTGCTACTA 841 TTGATCACAT ATTTGCCAGT AAAGCAGTGG TGAATGCCGC AATTCCAGCC TATCACCTAA 901 GAGACCTTAT CAAAAGCATG CTTCATGATG ATCCAAGCAG AAGAATTCCT GCTGAAATGG 961 CATTGTGCAG CCCATTCTTT AGCATTCCTT TTGCCCCTCA TATTGAAGAT CTGGTCATGC 1021 TTCCCACTCC AGTGCTAAGA CTGCTGAATG TGCTGGATGA TGATTATCTT GAGAATGAAG 1081 AGGAATATGA AGGTCTTTGT TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1141 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1201 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CCTCCACCGT 1261 CACTCCTGTC CTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1321 att