Construct: ORF TRCN0000488965
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021502.1_s317c1
- DNA Barcode:
- CTTCGTCATATACGTAGAGTGTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SCD5 (79966)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488965
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79966 | SCD5 | stearoyl-CoA desaturase 5 | NM_001037582.3 | 100% | 100% | |
| 2 | human | 79966 | SCD5 | stearoyl-CoA desaturase 5 | NM_024906.2 | 69.3% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1059
- ORF length:
- 990
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gccaggcccg gccaccgacg cggggaagat ccctttctgc gacgccaagg 121 aagaaatccg tgccgggctc gaaagctctg agggcggcgg cggcccggag aggccaggcg 181 cgcgcgggca gcggcagaac atcgtctgga ggaatgtcgt cctgatgagc ttgctccact 241 tgggggccgt gtactccctg gtgctcatcc ccaaagccaa gccactcact ctgctctggg 301 cctacttctg cttcctcctg gccgctctgg gtgtgacagc tggtgcccat cgcttgtgga 361 gccacaggtc ctaccgggcc aagctgcctc tgaggatatt tctggctgtc gccaactcca 421 tggctttcca gaatgacatc ttcgagtggt ccagggacca ccgagcccac cacaagtact 481 cagagacgga tgctgacccc cacaatgccc gccggggctt cttcttctcc catattgggt 541 ggctgtttgt tcgcaagcat cgagatgtta ttgagaaggg gagaaagctt gacgtcactg 601 acctgcttgc tgatcctgtg gtccggatcc agagaaagta ctataagatc tccgtggtgc 661 tcatgtgctt tgtggtcccc acgctggtgc cctggtacat ctggggagag agtctgtgga 721 attcctactt cttGGCCTCT ATTCTCCGCT ATACCATCTC ACTCAACATC AGCTGGCTGG 781 TCAACAGCGC CGCCCACATG TATGGAAACC GGCCCTATGA CAAGCACATC AGCCCTCGGC 841 AGAACCCACT CGTCGCTCTG GGTGCCATTG GTGAAGGCTT CCATAATTAC CATCACACCT 901 TTCCCTTTGA CTACTCTGCG AGTGAATTTG GCTTAAATTT TAACCCAACC ACCTGGTTCA 961 TTGATTTCAT GTGCTGGCTG GGGCTGGCCA CTGACCGCAA ACGGGCAACC AAGCCGATGA 1021 TCGAGGCCCG GAAGGCCAGG ACTGGAGACA GCAGTGCTTG AGACCCAGCT TTCTTGTACA 1081 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1141 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1201 AGGACGACTT CGTCATATAC GTAGAGTGTT AACGCGTTAA GTCgacaatc aacctctgga 1261 ttacaaaatt tgtgaaagat t