Construct: ORF TRCN0000488988
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021964.1_s317c1
- DNA Barcode:
- TCCTATTTTCCAACCCTGAGTTGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LAG3 (3902)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488988
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3902 | LAG3 | lymphocyte activating 3 | NM_002286.6 | 99.9% | 99.8% | 1364T>C |
| 2 | human | 3902 | LAG3 | lymphocyte activating 3 | XM_011520956.1 | 84.5% | 84.3% | 1054_1055ins243;1121T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1644
- ORF length:
- 1575
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtgggaggct cagttcctgg gcttgctgtt tctgcagccg ctttgggtgg 121 ctccagtgaa gcctctccag ccaggggctg aggtcccggt ggtgtgggcc caggaggggg 181 ctcctgccca gctcccctgc agccccacaa tccccctcca ggatctcagc cttctgcgaa 241 gagcaggggt cacttggcag catcagccag acagtggccc gcccgctgcc gcccccggcc 301 atcccctggc ccccggccct cacccggcgg cgccctcctc ctgggggccc aggccccgcc 361 gctacacggt gctgagcgtg ggtcccggag gcctgcgcag cgggaggctg cccctgcagc 421 cccgcgtcca gctggatgag cgcggccggc agcgcgggga cttctcgcta tggctgcgcc 481 cagcccggcg cgcggacgcc ggcgagtacc gcgccgcggt gcacctcagg gaccgcgccc 541 tctcctgccg cctccgtctg cgcctgggcc aggcctcgat gactgccagc cccccaggat 601 ctctcagagc ctccgactgg gtcattttga actgctcctt cagccgccct gaccgcccag 661 cctctgtgca ttggttccgg aaccggggcc agggccgagt ccctgtccgg gagtcccccc 721 atcaccactt agcggaaagc ttcctcttcc tgccccaagt cagccccatg gactctgggc 781 cctggggctg catcctcacc tacagagatg gcttcaacgt ctccatcatg tataacctca 841 ctgttctggg tctggagccc ccaactccct tgacagtgta cgctggagca ggttccaggg 901 tggggctgcc ctgccgcctg cctgctggtg tggggacccg gtctttcctc actgccaagt 961 ggactcctcc tgggggaggc cctgacctcc tggtgactgg agacaatggc gactttaccc 1021 ttcgactaga ggatgtgagc caggcccagg ctgggaccta cacctgccat atccatctgc 1081 aggaacagca gctcaatgcc actgtcacat tggcaatcat cacagtgact cccaaatcct 1141 ttgggtcacc tggatccctg gggaagctgc tttgtgaggt gactccagta tctggacaag 1201 aacgctttgt gtggagctct ctggacaccc catcccagag gagtttctca ggaccttggc 1261 tggaggcaca ggaggcccag ctcctttccc agccttGGCA ATGCCAGCTG TACCAGGGGG 1321 AGAGGCTTCT TGGAGCAGCA GTGTACTTCA CAGAGCTGTC TAGCCCAGGT GCCCAACGCT 1381 CTGGGAGAGC CCCAGGTGCC CTCCCAGCAG GCCACCTCCT GCTGTTTCTC ACCCTTGGTG 1441 TCCTTTCTCT GCTCCTTTTG GTGACTGGAG CCTTTGGCTT TCACCTTTGG AGAAGACAGT 1501 GGCGACCAAG ACGATTTTCT GCCTTAGAGC AAGGGATTCA CCCTCCGCAG GCTCAGAGCA 1561 AGATAGAGGA GCTGGAGCAA GAACCGGAGC CGGAGCCGGA GCCGGAACCG GAGCCCGAGC 1621 CCGAGCCCGA GCCGGAGCAG CTCTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1681 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1741 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCCTATTT 1801 TCCAACCCTG AGTTGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1861 aagatt