Construct: ORF TRCN0000489012
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019196.1_s317c1
- DNA Barcode:
- ACCTTGTCTTGTCCTCTTGACGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ROM1 (6094)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489012
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6094 | ROM1 | retinal outer segment membr... | NM_000327.3 | 99.8% | 100% | 669G>C;676C>T |
| 2 | mouse | 19881 | Rom1 | rod outer segment membrane ... | NM_009073.4 | 85.1% | 84.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1128
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggcg ccggtgttgc ccctggtgct gcccctgcag ccccgcatcc 121 gcctggcaca agggctctgg ctcctctcct ggctgctggc gctggctggt ggcgtcatcc 181 tcctctgtag tgggcacctc ctggtccagc taaggcacct tggcaccttc ctggctccct 241 cctgtcagtt ccctgtcctg ccccaggctg ccctggcagc gggcgcggtg gctctgggca 301 caggactagt gggtgtagga gccagccggg caagtctgaa tgcagctcta taccctccct 361 ggcgaggggt cctgggcccg ctgctggtgg ctggcacggc tggtgggggg gggctcctgg 421 tcgtcgccct cgggctagcc ctggctttgc ctgggagtct ggatgaggcg ctggaggagg 481 gcctggtgac tgccttggct cactacaagg acacagaggt gcctgggcac tgtcaggcca 541 aaaggctggt ggatgagctg caactgaggt accactgctg cgggcgccac gggtacaagg 601 attggtttgg ggtccagtgg gtcagcagcc gttacctgga tcccggtgac cgggatgtgg 661 ctgaccggat ccagagcaat gtagaaggcc tatacctgac tgatggggtc cctttctcct 721 gttgcaaccc ccactcaccc cgcccttgct tgcaaaaccg tctttcagac tcctacgccc 781 acccccTGTT CGATCCCCGA CAACCCAACC AAAACCTCTG GGCCCAAGGG TGCCATGAGG 841 TGCTGCTGGA GCACTTGCAG GACTTGGCAG GCACACTGGG TAGCATGCTG GCTGTCACCT 901 TCCTACTGCA GGCTCTGGTG CTCCTTGGCC TGCGGTACCT GCAAACAGCA CTGGAGGGGC 961 TTGGAGGGGT CATTGATGCG GGAGGAGAGA CCCAGGGCTA TCTCTTTCCC AGTGGGCTGA 1021 AAGATATGCT GAAAACAGCA TGGCTACAGG GAGGGGTTGC CTGCAGGCCA GCACCTGAGG 1081 AGGCCCCACC AGGAGAAGCA CCTCCCAAGG AGGATCTATC TGAGGCCGAC CCAGCTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGAACCTTGT CTTGTCCTCT TGACGAGACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt