Construct: ORF TRCN0000489028
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021189.1_s317c1
- DNA Barcode:
- TATCCTTAAAGGGCTTTTGCCCTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TM7SF3 (51768)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489028
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51768 | TM7SF3 | transmembrane 7 superfamily... | NM_016551.3 | 100% | 100% | |
| 2 | human | 51768 | TM7SF3 | transmembrane 7 superfamily... | XM_017019463.2 | 95% | 95% | 246_335del |
| 3 | human | 51768 | TM7SF3 | transmembrane 7 superfamily... | XM_005253391.4 | 91% | 91% | 1035_1036ins153 |
| 4 | human | 51768 | TM7SF3 | transmembrane 7 superfamily... | XM_017019464.2 | 82.1% | 82.1% | 90_91ins306 |
| 5 | human | 51768 | TM7SF3 | transmembrane 7 superfamily... | XM_011520690.3 | 78.5% | 78.5% | 0_1ins366 |
| 6 | human | 51768 | TM7SF3 | transmembrane 7 superfamily... | XM_017019465.2 | 73.1% | 73.1% | 90_91ins306;729_730ins153 |
| 7 | human | 51768 | TM7SF3 | transmembrane 7 superfamily... | XM_017019466.2 | 69.6% | 69.6% | 0_1ins366;669_670ins153 |
| 8 | human | 51768 | TM7SF3 | transmembrane 7 superfamily... | XM_011520691.2 | 52.9% | 44.3% | (many diffs) |
| 9 | human | 51768 | TM7SF3 | transmembrane 7 superfamily... | XR_001748764.2 | 18% | 1_226del;1095_1198del;2041_9484del | |
| 10 | human | 51768 | TM7SF3 | transmembrane 7 superfamily... | XR_931304.3 | 17.1% | 1_226del;1415_1416ins98;1839_9280del | |
| 11 | human | 51768 | TM7SF3 | transmembrane 7 superfamily... | XR_931305.3 | 16.9% | (many diffs) | |
| 12 | human | 51768 | TM7SF3 | transmembrane 7 superfamily... | XR_001748763.2 | 15.9% | (many diffs) | |
| 13 | human | 51768 | TM7SF3 | transmembrane 7 superfamily... | XR_242895.4 | 15.5% | 1_226del;1262_1263ins251;1686_9127del | |
| 14 | human | 51768 | TM7SF3 | transmembrane 7 superfamily... | XR_002957337.1 | 15.3% | (many diffs) | |
| 15 | human | 51768 | TM7SF3 | transmembrane 7 superfamily... | XR_001748762.2 | 15.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1782
- ORF length:
- 1710
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggggttc ctgcagctgc tggtcgtagc ggtgctggca tccgaacacc 121 gggtggctgg tgcagccgag gtcttcggga attccagcga gggtcttatt gaattttctg 181 tggggaaatt tagatacttc gagctcaata ggccctttcc agaggaagct attttgcatg 241 atatttcaag caatgtgact tttcttattt tccaaataca ctcacagtat cagaatacaa 301 ctgtttcctt ttctccgact ctcctttcca attcctcgga aacaggcact gccagtggac 361 tggttttcat ccttagacca gagcagagta catgcacttg gtacttgggg acttcaggca 421 tacagcctgt ccagaatatg gctatcctac tctcctactc agaaagagat cctgtccctg 481 gaggctgtaa tttggagttc gatttagata ttgatcccaa catttacttg gagtataatt 541 tctttgaaac gactatcaag tttgccccag caaacctagg ctatgcgaga ggcgtagatc 601 ccccaccatg tgacgctggg acagaccagg actccaggtg gaggttgcag tatgatgtct 661 atcagtattt tctgcctgag aatgacctca ctgaggagat gttgctgaag catctgcaga 721 ggatggtcag tgtgccccag gtgaaggcca gtgctctcaa ggtggttacc ctaacagcta 781 atgataagac aagtgtttcc ttctcctccc tcccgggaca aggtgtcata tacaatgtca 841 ttgtttggga cccgtttcta aatacatctg ctgcctacat tcctgctcac acatacgctt 901 gcagctttga ggcaggagag ggtagttgtg cttccctagg aagagtgtct tccaaagtgt 961 tcttcactct ttttgccctg cttggtttct tcatttgttt ctttggacac agattctgga 1021 aaacagaatt attcttcata ggctttatca tcatgggatt cttcttttat atactgatta 1081 caagactgac acctatcaag tatgatgtga atctgattct gacagctgtc actggaagcg 1141 tcggtggaat gttcttggta gctgtgtggt ggcgatttgg aatcctctcg atctgcatgc 1201 tctgtgttgg actagtgctg gggttcctca tctcgtcagt gactttcttt actccactgg 1261 gaaacctaaa gatttttcat gatgatggtg tattctgggt cactttctct tgcatagcta 1321 tcctcattcc agtagttttc atgggctgcc taagaatact gaacatactg acttgtggag 1381 tcattggctc ctattcggtg gttttagcca ttgacagtta ctggtccaca agCCTTTCCT 1441 ACATCACTTT GAACGTACTC AAGAGAGCGC TCAACAAGGA TTTCCACAGA GCTTTCACAA 1501 ATGTGCCTTT TCAAACTAAT GACTTCATTA TCCTGGCAGT ATGGGGCATG CTGGCTGTAA 1561 GTGGAATTAC GTTACAGATT CGAAGAGAGA GAGGACGACC GTTCTTCCCT CCCCACCCAT 1621 ACAAGTTATG GAAGCAAGAG AGAGAGCGCC GAGTGACAAA CATTCTGGAC CCTAGCTACC 1681 ACATTCCTCC ATTGAGAGAG AGGCTCTATG GCCGATTAAC CCAGATTAAA GGGCTCTTCC 1741 AGAAGGAGCA GCCAGCTGGA GAGAGAACGC CTTTGCTTCT GTAGGACCCA GCTTTCTTGT 1801 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1861 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1921 GAAAGGACGA TATCCTTAAA GGGCTTTTGC CCTGACGCGT TAAGTCgaca atcaacctct 1981 ggattacaaa atttgtgaaa gatt