Construct: ORF TRCN0000489030
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021141.1_s317c1
- DNA Barcode:
- TTGGTCGTCAATAACACTGGGCCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R20 (259295)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489030
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 259295 | TAS2R20 | taste 2 receptor member 20 | NM_176889.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 999
- ORF length:
- 927
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgatgagt tttctacaca ttgttttttc cattctagta gtggttgcat 121 ttattcttgg aaattttgcc aatggcttta tagcactgat aaatttcatt gcctgggtca 181 agagacaaaa gatctcctca gctgatcaaa ttattgctgc tctggcagtc tccagagttg 241 gtttgctctg ggtaatatta ttacattggt attcaactgt gttgaatcca acttcatcta 301 atttaaaagt aataattttt atttctaatg cctgggcagt aaccaatcat ttcagcatct 361 ggcttgctac tagcctcagc atattttatt tgctcaagat cgtcaatttc tccagactta 421 tttttcatca cttaaaaagg aaggctaaga gtgtagttct ggtgatagtg ttggggtctt 481 tgttcttttt ggtttgtcac cttgtgatga aacacacgta tataaatgtg tggacagaag 541 aatgtgaagg aaacgtaact tggaagatca aactgaggaa tgcaatgcac ctttccaact 601 tgactgtagc catgctagca aacttgatac cattcactct gacccTGATA TCTTTTCTGC 661 TGTTAATCTA CTCTCTGTGT AAACATCTGA AGAAGATGCA GCTCCATGGC AAAGGATCTC 721 AAGATCCCAG CACCAAGATC CACATAAAAG CTCTGCAAAC TGTGACCTCC TTCCTCATAT 781 TACTTGCCAT TTACTTTCTG TGTCTAATCA TATCGTTTTG GAATTTTAAG ATGCGACCAA 841 AAGAAATTGT CTTAATGCTT TGCCAAGCTT TTGGAATCAT ATATCCATCA TTCCACTCAT 901 TCATTCTGAT TTGGGGGAAC AAGACGCTAA AGCAGACCTT TCTTTCAGTT TTGTGGCAGG 961 TGACTTGCTG GGCAAAAGGA CAGAACCAGT CAACTCCATA GAACCCAGCT TTCTTGTACA 1021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1141 AGGACGATTG GTCGTCAATA ACACTGGGCC TACGCGTTAA GTCgacaatc aacctctgga 1201 ttacaaaatt tgtgaaagat t