Construct: ORF TRCN0000489034
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020920.1_s317c1
- DNA Barcode:
- CGAGCAGATGTGATTCAGGAGTCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TACR2 (6865)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489034
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6865 | TACR2 | tachykinin receptor 2 | NM_001057.3 | 99.9% | 99.7% | 734T>A |
| 2 | mouse | 21337 | Tacr2 | tachykinin receptor 2 | NM_009314.4 | 82.2% | 82.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1266
- ORF length:
- 1194
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggggacc tgtgacattg tgactgaagc caatatctca tctggccctg 121 agagcaacac cacgggcatc acagccttct ccatgcccag ctggcaactg gcactgtggg 181 ccacagccta cctggccctg gtgctggtgg ccgtgacggg taatgccatc gtcatctgga 241 tcatcctggc ccatcggagg atgcgcacag tcaccaacta cttcatcgtc aatctggcgc 301 tggctgacct ctgcatggct gccttcaatg ccgccttcaa ctttgtctat gccagccaca 361 acatctggta ctttggccgt gccttctgct acttccagaa cctcttcccc atcacagcca 421 tgtttgtcag catctactcc atgaccgcca ttgctgccga caggtacatg gccatcgtcc 481 accccttcca gcctcggctt tcagctccca gcaccaaggc ggttattgct ggcatctggc 541 tggtggctct cgccctggcc tcccctcagt gcttctactc caccgtcacc atggaccagg 601 gtgccaccaa gtgcgtggtg gcctggcccg aagacagcgg gggcaagacg ctcctcctgt 661 accacctcgt ggtgatcgcc ctcatctact tcctgccgct cgcggtgatg tttgtagcct 721 acagcgtcat cggcctcacg ctctggaggc gcgcagtgcc cggacatcag gcgcacggtg 781 ccaacctgcg ccacctgcag gccaagaaga agtttgtgaa gaccatggtg ctggtggtgc 841 tgacgtttgc catctgctgg ctgccctacc acctctactt catcctgggc agcttccagg 901 aggacatcta ctgccacaag ttcatccagc aagtctacct ggcactcttc tggttggcca 961 tgagctctac catgtacaat cccatcatct actgctgtct caaccacagg tttcgctctg 1021 gattccggct tgccTTCCGC TGCTGCCCAT GGGTCACACC CACCAAGGAA GATAAGCTCG 1081 AGCTGACTCC CACGACCTCC CTCTCCACGA GAGTCAACAG GTGTCACACT AAGGAGACTT 1141 TGTTCATGGC TGGGGACACA GCCCCCTCCG AGGCTACCAG TGGGGAGGCG GGGCGTCCCC 1201 AGGATGGATC AGGGCTATGG TTTGGGTATG GTTTGCTTGC CCCCACCAAA ACTCATGTTG 1261 AAATTTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1321 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1381 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGAGCA GATGTGATTC AGGAGTCGAC 1441 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt