Construct: ORF TRCN0000489040
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020741.1_s317c1
- DNA Barcode:
- TGCGGTCCCCACATTGGACTAAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PIK3CA (5290)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489040
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5290 | PIK3CA | phosphatidylinositol-4,5-bi... | NM_006218.2:c.1624G>A | 99.9% | 100% | 10C>A |
| 2 | human | 5290 | PIK3CA | phosphatidylinositol-4,5-bi... | NM_006218.4 | 99.9% | 99.9% | 10C>A;1624G>A |
| 3 | human | 5290 | PIK3CA | phosphatidylinositol-4,5-bi... | XM_006713658.4 | 99.9% | 99.9% | 10C>A;1624G>A |
| 4 | human | 5290 | PIK3CA | phosphatidylinositol-4,5-bi... | XM_011512894.2 | 99.9% | 99.9% | 10C>A;1624G>A |
| 5 | human | 5290 | PIK3CA | phosphatidylinositol-4,5-bi... | NM_006218.2:c.3204_3205insA | 99.9% | 99.6% | 10C>A;1624G>A;3205delA |
| 6 | mouse | 18706 | Pik3ca | phosphatidylinositol-4,5-bi... | NM_008839.2 | 89.5% | 98.5% | (many diffs) |
| 7 | mouse | 18706 | Pik3ca | phosphatidylinositol-4,5-bi... | XM_006535409.3 | 89.5% | 98.5% | (many diffs) |
| 8 | mouse | 18706 | Pik3ca | phosphatidylinositol-4,5-bi... | XM_006535410.3 | 89.5% | 98.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3276
- ORF length:
- 3204
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcctcca agaccatcat caggtgaact gtggggcatc cacttgatgc 121 ccccaagaat cctagtagaa tgtttactac caaatggaat gatagtgact ttagaatgcc 181 tccgtgaggc tacattaata accataaagc atgaactatt taaagaagca agaaaatacc 241 ccctccatca acttcttcaa gatgaatctt cttacatttt cgtaagtgtt actcaagaag 301 cagaaaggga agaatttttt gatgaaacaa gacgactttg tgaccttcgg ctttttcaac 361 cctttttaaa agtaattgaa ccagtaggca accgtgaaga aaagatcctc aatcgagaaa 421 ttggttttgc tatcggcatg ccagtgtgtg aatttgatat ggttaaagat ccagaagtac 481 aggacttccg aagaaatatt ctgaacgttt gtaaagaagc tgtggatctt agggacctca 541 attcacctca tagtagagca atgtatgtct atcctccaaa tgtagaatct tcaccagaat 601 tgccaaagca catatataat aaattagata aagggcaaat aatagtggtg atctgggtaa 661 tagtttctcc aaataatgac aagcagaagt atactctgaa aatcaaccat gactgtgtac 721 cagaacaagt aattgctgaa gcaatcagga aaaaaactcg aagtatgttg ctatcctctg 781 aacaactaaa actctgtgtt ttagaatatc agggcaagta tattttaaaa gtgtgtggat 841 gtgatgaata cttcctagaa aaatatcctc tgagtcagta taagtatata agaagctgta 901 taatgcttgg gaggatgccc aatttgatgt tgatggctaa agaaagcctt tattctcaac 961 tgccaatgga ctgttttaca atgccatctt attccagacg catttccaca gctacaccat 1021 atatgaatgg agaaacatct acaaaatccc tttgggttat aaatagtgca ctcagaataa 1081 aaattctttg tgcaacctac gtgaatgtaa atattcgaga cattgataag atctatgttc 1141 gaacaggtat ctaccatgga ggagaaccct tatgtgacaa tgtgaacact caaagagtac 1201 cttgttccaa tcccaggtgg aatgaatggc tgaattatga tatatacatt cctgatcttc 1261 ctcgtgctgc tcgactttgc ctttccattt gctctgttaa aggccgaaag ggtgctaaag 1321 aggaacactg tccattggca tggggaaata taaacttgtt tgattacaca gacactctag 1381 tatctggaaa aatggctttg aatctttggc cagtacctca tggattagaa gatttgctga 1441 accctattgg tgttactgga tcaaatccaa ataaagaaac tccatgctta gagttggagt 1501 ttgactggtt cagcagtgtg gtaaagttcc cagatatgtc agtgattgaa gagcatgcca 1561 attggtctgt atcccgagaa gcaggattta gctattccca cgcaggactg agtaacagac 1621 tagctagaga caatgaatta agggaaaatg acaaagaaca gctcaaagca atttctacac 1681 gagatcctct ctctaaaatc actgagcagg agaaagattt tctatggagt cacagacact 1741 attgtgtaac tatccccgaa attctaccca aattgcttct gtctgttaaa tggaattcta 1801 gagatgaagt agcccagatg tattgcttgg taaaagattg gcctccaatc aaacctgaac 1861 aggctatgga acttctggac tgtaattacc cagatcctat ggttcgaggt tttgctgttc 1921 ggtgcttgga aaaatattta acagatgaca aactttctca gtatttaatt cagctagtac 1981 aggtcctaaa atatgaacaa tatttggata acttgcttgt gagattttta ctgaagaaag 2041 cattgactaa tcaaaggatt gggcactttt tcttttggca tttaaaatct gagatgcaca 2101 ataaaacagt tagccagagg tttggcctgc ttttggagtc ctattgtcgt gcatgtggga 2161 tgtatttgaa gcacctgaat aggcaagtcg aggcaatgga aaagctcatt aacttaactg 2221 acattctcaa acaggagaag aaggatgaaa cacaaaaggt acagatgaag tttttagttg 2281 agcaaatgag gcgaccagat ttcatggatg ctctacaggg ctttctgtct cctctaaacc 2341 ctgctcatca actaggaaac ctcaggcttg aagagtgtcg aattatgtcc tctgcaaaaa 2401 ggccactgtg gttgaattgg gagaacccag acatcatgtc agagttactg tttcagaaca 2461 atgagatcat ctttaaaaat ggggatgatt tacggcaaga tatgctaaca cttcaaatta 2521 ttcgtattat ggaaaatatc tggcaaaatc aaggtcttga tcttcgaatg ttaccttatg 2581 gttgtctgtc aatcggtgac tgtgtgggac ttattgaggt ggtgcgaaat tctcacacta 2641 ttatgcaaat tcagtgcaaa ggcggcttga aaggtgcact gcagttcaac agccacacac 2701 tacatcagtg gctcaaagac aagaacaaag gagaaatata tgatgcagcc attgacctgt 2761 ttacacgttc atgtgctgga tactgtgtag ctaccttcat tttgggaatt ggagatcgtc 2821 acaatagtaa catcatggtg aaagacgatg gacaactgtt tcatatagat tttgGACACT 2881 TTTTGGATCA CAAGAAGAAA AAATTTGGTT ATAAACGAGA ACGTGTGCCA TTTGTTTTGA 2941 CACAGGATTT CTTAATAGTG ATTAGTAAAG GAGCCCAAGA ATGCACAAAG ACAAGAGAAT 3001 TTGAGAGGTT TCAGGAGATG TGTTACAAGG CTTATCTAGC TATTCGACAG CATGCCAATC 3061 TCTTCATAAA TCTTTTCTCA ATGATGCTTG GCTCTGGAAT GCCAGAACTA CAATCTTTTG 3121 ATGACATTGC ATACATTCGA AAGACCCTAG CCTTAGATAA AACTGAGCAA GAGGCTTTGG 3181 AGTATTTCAT GAAACAAATG AATGATGCAC ATCATGGTGG CTGGACAACA AAAATGGATT 3241 GGATCTTCCA CACAATTAAA CAGCATGCAT TGAACTGAGA CCCAGCTTTC TTGTACAAAG 3301 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 3361 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 3421 ACGATGCGGT CCCCACATTG GACTAAGAAC GCGTTAAGTC gacaatcaac ctctggatta 3481 caaaatttgt gaaagatt