Construct: ORF TRCN0000489063
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021212.1_s317c1
- DNA Barcode:
- AGCGGGTCAATTATGCTTTGTAAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HOMER1 (9456)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489063
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9456 | HOMER1 | homer scaffold protein 1 | NM_004272.5 | 100% | 100% | |
| 2 | human | 9456 | HOMER1 | homer scaffold protein 1 | NM_001277077.1 | 63.2% | 63.2% | 294_295ins390 |
| 3 | human | 9456 | HOMER1 | homer scaffold protein 1 | XM_017010059.2 | 50.7% | 49.5% | (many diffs) |
| 4 | human | 9456 | HOMER1 | homer scaffold protein 1 | NM_001277078.1 | 49.8% | 49.4% | 528_529delGAinsTTC;531_532ins530 |
| 5 | mouse | 26556 | Homer1 | homer scaffolding protein 1 | NM_001284189.2 | 91.1% | 98.5% | (many diffs) |
| 6 | mouse | 26556 | Homer1 | homer scaffolding protein 1 | NM_147176.4 | 88.1% | 95.3% | (many diffs) |
| 7 | mouse | 26556 | Homer1 | homer scaffolding protein 1 | NM_152134.3 | 87% | 93.7% | (many diffs) |
| 8 | mouse | 26556 | Homer1 | homer scaffolding protein 1 | NM_001347598.1 | 50.3% | 50.4% | (many diffs) |
| 9 | mouse | 26556 | Homer1 | homer scaffolding protein 1 | NM_011982.4 | 47.8% | 49.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1134
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggggaa caacctatct tcagcactcg agctcatgtc ttccaaattg 121 acccaaacac aaagaagaac tgggtaccca ccagcaagca tgcagttact gtgtcttatt 181 tctatgacag cacaagaaat gtgtatagga taatcagttt agatggctca aaggcaataa 241 taaatagtac catcacccca aacatgacat ttactaaaac atctcagaag tttggccagt 301 gggctgatag ccgggcaaac accgtttatg gattgggatt ctcctctgag catcatcttt 361 cgaaatttgc agaaaagttt caggaattta aagaagctgc tcgactagca aaggaaaaat 421 cacaagagaa gatggaactt accagtacac cttcacagga atccgcaggc ggggatcttc 481 agtctccttt aacaccggaa agtatcaacg ggacagatga tgaaagaaca cctgatgtga 541 cacagaactc agagccaagg gctgaaccaa ctcagaatgc attgccattt tcacatagtt 601 cagcaatcag caaacattgg gaggctgaac tggctaccct caaaggaaat aatgccaaac 661 tcactgcagc cctgctggag tccactgcca atgtgaaaca atggaaacag caacttgctg 721 cctatcaaga ggaagcagaa cgtctgcaca agcgggTGAC TGAACTTGAA TGTGTTAGTA 781 GCCAAGCAAA TGCAGTACAT ACTCATAAGA CAGAATTAAA TCAGACAATA CAAGAACTGG 841 AAGAGACACT GAAACTGAAG GAAGAGGAAA TAGAAAGGTT AAAACAAGAA ATTGATAATG 901 CCAGAGAACT ACAAGAACAG AGGGATTCTT TGACTCAGAA ACTACAGGAA GTAGAAATTC 961 GGAACAAAGA CCTGGAGGGA CAACTGTCTG ACTTAGAGCA ACGTCTGGAG AAAAGTCAGA 1021 ATGAACAAGA AGCTTTTCGC AATAACCTGA AGACACTCTT AGAAATTCTG GATGGAAAGA 1081 TATTTGAACT AACAGAATTA CGAGATAACT TGGCCAAGCT ACTAGAATGC AGCTAGAACC 1141 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GAAGCGGGTC AATTATGCTT TGTAAAACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt