Construct: ORF TRCN0000489079

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020236.1_s317c1
DNA Barcode:
TGGCGGATGTTTTCTCTCGCCGTT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PDXK (8566)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489079
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8566 PDXK pyridoxal kinase NM_003681.5 99.8% 100% 510G>T
2 human 8566 PDXK pyridoxal kinase XM_005261196.3 90.7% 86.4% (many diffs)
3 human 8566 PDXK pyridoxal kinase NM_001331030.2 86.8% 85.2% (many diffs)
4 human 8566 PDXK pyridoxal kinase XM_011529758.3 81.6% 73.7% (many diffs)
5 human 8566 PDXK pyridoxal kinase XM_005261199.2 76.4% 76.6% 0_1ins219;291G>T
6 human 8566 PDXK pyridoxal kinase XM_011529760.2 76.4% 76.6% 0_1ins219;291G>T
7 human 8566 PDXK pyridoxal kinase XM_011529761.1 76.4% 76.6% 0_1ins219;291G>T
8 human 8566 PDXK pyridoxal kinase XM_017028483.2 76.4% 76.6% 0_1ins219;291G>T
9 human 8566 PDXK pyridoxal kinase XM_017028484.1 76.4% 76.6% 0_1ins219;291G>T
10 human 8566 PDXK pyridoxal kinase XM_005261195.2 74.7% 70.7% (many diffs)
11 mouse 216134 Pdxk pyridoxal (pyridoxine, vita... NM_172134.2 84.7% 86.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1008
ORF length:
936
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggaggag gagtgccggg tgctctccat acagagccac gtcatccgcg
121gctacgtggg caaccgggcg gccacgttcc cgctgcaggt tttgggattt gagattgacg
181cggtgaactc tgtccagttt tcaaaccaca caggctatgc ccactggaag ggccaagtgc
241tgaattcaga tgagctccag gagttgtacg aaggcctgag gctgaacaac atgaataaat
301atgactacgt gctcacaggt tatacgaggg acaagtcgtt cctggccatg gtggtggaca
361ttgtgcagga gctgaagcag cagaacccca ggctggtgta cgtgtgtgat ccagtcttgg
421gtgacaagtg ggacggcgaa ggctcgatgt acgtcccgga ggacctcctt cccgtctaca
481aagaaaaagt ggtgccgctt gcagacatta tcacgcccaa ccagtttgag gccgagttac
541tgagtggccg gaagatccac agccaggagg aagccttgcg tgtgatggac atgctgcact
601ctatgggccc cgacaccgtg gtcatcacca gctccgacct gccctccccg cagggcagca
661actacctgat tgtgctggGG AGTCAGAGGA GGAGGAATCC CGCTGGCTCC GTGGTGATGG
721AACGCATCCG GATGGACATT CGCAAAGTGG ACGCCGTCTT TGTGGGCACT GGGGACCTGT
781TTGCTGCCAT GCTCCTGGCG TGGACACACA AGCACCCCAA TAACCTCAAG GTGGCCTGTG
841AGAAGACCGT GTCTACCTTG CACCACGTTC TGCAGAGGAC CATCCAGTGT GCAAAAGCCC
901AGGCCGGGGA AGGAGTGAGG CCCAGCCCCA TGCAGCTGGA GCTGCGGATG GTGCAGAGCA
961AAAGGGACAT CGAGGACCCA GAGATCGTCG TCCAGGCCAC GGTGCTGTGA GACCCAGCTT
1021TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1081CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1141CTTGTGGAAA GGACGATGGC GGATGTTTTC TCTCGCCGTT ACGCGTTAAG TCgacaatca
1201acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)