Construct: ORF TRCN0000489172
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019556.1_s317c1
- DNA Barcode:
- CTTCGTGACAATACCTTTCGGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDK2 (1017)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489172
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1017 | CDK2 | cyclin dependent kinase 2 | NM_001798.5 | 100% | 100% | |
| 2 | human | 1017 | CDK2 | cyclin dependent kinase 2 | NM_052827.4 | 88.5% | 88.5% | 485_486ins102 |
| 3 | human | 1017 | CDK2 | cyclin dependent kinase 2 | XM_011537732.2 | 86.1% | 86.1% | 589_732del |
| 4 | human | 1017 | CDK2 | cyclin dependent kinase 2 | NM_001290230.2 | 79.8% | 79.8% | 116_117ins78;407_408ins102 |
| 5 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | NM_016756.4 | 92.5% | 98.9% | (many diffs) |
| 6 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | NM_183417.3 | 79.6% | 85.2% | (many diffs) |
| 7 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | XM_006513162.2 | 55.7% | 59.5% | (many diffs) |
| 8 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | XM_011243321.2 | 55.7% | 59.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 987
- ORF length:
- 894
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgga tcctggcgct tcatggagaa cttccaaaag gtggaaaaga 121 tcggagaggg cacgtacgga gttgtgtaca aagccagaaa caagttgacg ggagaggtgg 181 tggcgcttaa gaaaatccgc ctggacactg agactgaggg tgtgcccagt actgccatcc 241 gagagatctc tctgcttaag gagcttaacc atcctaatat tgtcaagctg ctggatgtca 301 ttcacacaga aaataaactc tacctggttt ttgaatttct gcaccaagat ctcaagaaat 361 tcatggatgc ctctgctctc actggcattc ctcttcccct catcaagagc tatctgttcc 421 agctgctcca gggcctagct ttctgccatt ctcatcgggt cctccaccga gaccttaaac 481 ctcagaatct gcttattaac acagaggggg ccatcaagct agcagacttt ggactagcca 541 gagcttttgg agtccctgtt cgtacttaca cccatgaggt ggtgaccctg tggtaccgag 601 ctcctgaaat cctcctgggc tgcaaatatt attccacagc tgtggacatc tggagcctgg 661 gctgcatctt tgctgagatg gtgactcgcc gggCCCTATT CCCTGGAGAT TCTGAGATTG 721 ACCAGCTCTT CCGGATCTTT CGGACTCTGG GGACCCCAGA TGAGGTGGTG TGGCCAGGAG 781 TTACTTCTAT GCCTGATTAC AAGCCAAGTT TCCCCAAGTG GGCCCGGCAA GATTTTAGTA 841 AAGTTGTACC TCCCCTGGAT GAAGATGGAC GGAGCTTGTT ATCGCAAATG CTGCACTACG 901 ACCCTAACAA GCGGATTTCG GCCAAGGCAG CCCTGGCTCA CCCTTTCTTC CAGGATGTGA 961 CCAAGCCAGT ACCCCATCTT CGACTCAAGG GTGGGCGCGC CGACCCAGCT TTCTTGTACA 1021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1141 AGGACGACTT CGTGACAATA CCTTTCGGAA TACGCGTTAA GTCgacaatc aacctctgga 1201 ttacaaaatt tgtgaaagat t