Construct: ORF TRCN0000489240
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020858.1_s317c1
- DNA Barcode:
- AGTATTACCCGCCGCGGTCATCAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- F2RL1 (2150)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489240
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2150 | F2RL1 | F2R like trypsin receptor 1 | NM_005242.6 | 99.8% | 99.7% | 89G>A;621T>C |
| 2 | human | 2150 | F2RL1 | F2R like trypsin receptor 1 | XM_017009223.1 | 93.1% | 92.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1263
- ORF length:
- 1191
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcggagc cccagcgcgg cgtggctgct gggggccgcc atcctgctag 121 cagcctctct ctcctgcagt ggcaccatcc aaggaaccaa tagatcctct aaaggaagaa 181 gccttattgg taaggttgat ggcacatccc acgtcactgg aaaaggagtt acagttgaaa 241 cagtcttttc tgtggatgag ttttctgcat ctgtcctcac tggaaaactg accactgtct 301 tccttccaat tgtctacaca attgtgtttg tggtgggttt gccaagtaac ggcatggccc 361 tgtgggtctt tcttttccga actaagaaga agcaccctgc tgtgatttac atggccaatc 421 tggccttggc tgacctcctc tctgtcatct ggttcccctt gaagattgcc tatcacatac 481 atggcaacaa ctggatttat ggggaagctc tttgtaatgt gcttattggc tttttctatg 541 gcaacatgta ctgttccatt ctcttcatga cctgcctcag tgtgcagagg tattgggtca 601 tcgtgaaccc catggggcac tccaggaaga aggcaaacat tgccattggc atctccctgg 661 caatatggct gctgattctg ctggtcacca tccctttgta tgtcgtgaag cagaccatct 721 tcattcctgc cctgaacatc acgacctgtc atgatgtttt gcctgagcag ctcttggtgg 781 gagacatgtt caattacttc ctctctctgg ccattggggt ctttctgttc ccagccttcc 841 tcacagcctc tgcctatgtg ctgatgatca gaatgctgcg atcttctgcc atggatgaaa 901 actcagagaa gaaaaggaag agggccatca aactcattgt cactgtcctg gccatgtacc 961 TGATCTGCTT CACTCCTAGT AACCTTCTGC TTGTGGTGCA TTATTTTCTG ATTAAGAGCC 1021 AGGGCCAGAG CCATGTCTAT GCCCTGTACA TTGTAGCCCT CTGCCTCTCT ACCCTTAACA 1081 GCTGCATCGA CCCCTTTGTC TATTACTTTG TTTCACATGA TTTCAGGGAT CATGCAAAGA 1141 ACGCTCTCCT TTGCCGAAGT GTCCGCACTG TAAAGCAGAT GCAAGTATCC CTCACCTCAA 1201 AGAAACACTC CAGGAAATCC AGCTCTTACT CTTCAAGTTC AACCACTGTT AAGACCTCCT 1261 ATTAGGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1321 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1381 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGTATTACC CGCCGCGGTC ATCAGACGCG 1441 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt