Construct: ORF TRCN0000489300
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019462.1_s317c1
- DNA Barcode:
- TGGCGAAGTCCACGCAACGACAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC2A2 (6514)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489300
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6514 | SLC2A2 | solute carrier family 2 mem... | NM_000340.2 | 99.9% | 99.8% | 1572_1573insG |
| 2 | human | 6514 | SLC2A2 | solute carrier family 2 mem... | XM_011513087.2 | 95% | 92.1% | (many diffs) |
| 3 | human | 6514 | SLC2A2 | solute carrier family 2 mem... | NM_001278658.2 | 76.8% | 76.5% | (many diffs) |
| 4 | human | 6514 | SLC2A2 | solute carrier family 2 mem... | NM_001278659.2 | 66.9% | 66.8% | 0_1ins519;1053_1054insG |
| 5 | human | 6514 | SLC2A2 | solute carrier family 2 mem... | XM_024453720.1 | 66.9% | 66.8% | 0_1ins519;1053_1054insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1641
- ORF length:
- 1575
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaggat 61 ccaccatgac agaagataag gtcactggga ccctggtttt cactgtcatc actgctgtgc 121 tgggttcctt ccagtttgga tatgacattg gtgtgatcaa tgcacctcaa caggtaataa 181 tatctcacta tagacatgtt ttgggtgttc cactggatga ccgaaaagct atcaacaact 241 atgttatcaa cagtacagat gaactgccca caatctcata ctcaatgaac ccaaaaccaa 301 ccccttgggc tgaggaagag actgtggcag ctgctcaact aatcaccatg ctctggtccc 361 tgtctgtatc cagctttgca gttggtggaa tgactgcatc attctttggt gggtggcttg 421 gggacacact tggaagaatc aaagccatgt tagtagcaaa cattctgtca ttagttggag 481 ctctcttgat ggggttttca aaattgggac catctcatat acttataatt gctggaagaa 541 gcatatcagg actatattgt gggctaattt caggcctggt tcctatgtat atcggtgaaa 601 ttgctccaac cgctctcagg ggagcacttg gcacttttca tcagctggcc atcgtcacgg 661 gcattcttat tagtcagatt attggtcttg aatttatctt gggcaattat gatctgtggc 721 acatcctgct tggcctgtct ggtgtgcgag ccatccttca gtctctgcta ctctttttct 781 gtccagaaag ccccagatac ctttacatca agttagatga ggaagtcaaa gcaaaacaaa 841 gcttgaaaag actcagagga tatgatgatg tcaccaaaga tattaatgaa atgagaaaag 901 aaagagaaga agcatcgagt gagcagaaag tctctataat tcagctcttc accaattcca 961 gctaccgaca gcctattcta gtggcactga tgctgcatgt ggctcagcaa ttttccggaa 1021 tcaatggcat tttttactac tcaaccagca tttttcagac ggctggtatc agcaaacctg 1081 tttatgcaac cattggagtt ggcgctgtaa acatggtttt cactgctgtc tctgtattcc 1141 ttgtggagaa ggcagggcga cgttctctct ttctaattgg aatgagtggg atgtttgttt 1201 gtgccatctt catgtcagtg ggacttgtgc tgctgaataa gttctcttgg atgagttatg 1261 tgagcatgat agccatcttc ctctttgtca gcttctttga aattgggcca ggcccgatcc 1321 ccTGGTTCAT GGTGGCTGAG TTTTTCAGTC AAGGACCACG TCCTGCTGCT TTAGCAATAG 1381 CTGCATTCAG CAATTGGACC TGCAATTTCA TTGTAGCTCT GTGTTTCCAG TACATTGCGG 1441 ACTTCTGTGG ACCTTATGTG TTTTTCCTCT TTGCTGGAGT GCTCCTGGCC TTTACCCTGT 1501 TCACATTTTT TAAAGTTCCA GAAACCAAAG GAAAGTCTTT TGAGGAAATT GCTGCAGAAT 1561 TCCAAAAGAA GAGTGGCTCA GCCCACAGGC CAAAAGCTGC TGTAGAAATG AAATTCCTAG 1621 GAGCTACAGA GACTGTGGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1681 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1741 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGGCGAA GTCCACGCAA 1801 CGACAACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt