Construct: ORF TRCN0000489304
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021853.1_s317c1
- DNA Barcode:
- TATTGACCAATCTTAGATATTTGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MCM2 (4171)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489304
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4171 | MCM2 | minichromosome maintenance ... | NM_004526.4 | 100% | 100% | |
| 2 | human | 4171 | MCM2 | minichromosome maintenance ... | XM_024453531.1 | 99% | 99% | 0_1ins27 |
| 3 | human | 4171 | MCM2 | minichromosome maintenance ... | NR_073375.2 | 78.5% | 1_56del;468_486del;2788_3453del | |
| 4 | mouse | 17216 | Mcm2 | minichromosome maintenance ... | NM_008564.2 | 87.6% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2781
- ORF length:
- 2712
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcggaatca tcggaatcct tcaccatggc atccagcccg gcccagcgtc 121 ggcgaggcaa tgatcctctc acctccagcc ctggccgaag ctcccggcgt actgatgccc 181 tcacctccag ccctggccgt gaccttccac catttgagga tgagtccgag gggctcctag 241 gcacagaggg gcccctggag gaagaagagg atggagagga gctcattgga gatggcatgg 301 aaagggacta ccgcgccatc ccagagctgg acgcctatga ggccgaggga ctggctctgg 361 atgatgagga cgtagaggag ctgacggcca gtcagaggga ggcagcagag cgggccatgc 421 ggcagcgtga ccgggaggct ggccggggcc tgggccgcat gcgccgtggg ctcctgtatg 481 acagcgatga ggaggacgag gagcgccctg cccgcaagcg ccgccaggtg gagcgggcca 541 cggaggacgg cgaggaggac gaggagatga tcgagagcat cgagaacctg gaggatctca 601 aaggccactc tgtgcgcgag tgggtgagca tggcgggccc ccggctggag atccaccacc 661 gcttcaagaa cttcctgcgc actcacgtcg acagccacgg ccacaacgtc ttcaaggagc 721 gcatcagcga catgtgcaaa gagaaccgtg agagcctggt ggtgaactat gaggacttgg 781 cagccaggga gcacgtgctg gcctacttcc tgcctgaggc accggcggag ctgctgcaga 841 tctttgatga ggctgccctg gaggtggtac tggccatgta ccccaagtac gaccgcatca 901 ccaaccacat ccatgtccgc atctcccacc tgcctctggt ggaggagctg cgctcgctga 961 ggcagctgca tctgaaccag ctgatccgca ccagtggggt ggtgaccagc tgcactggcg 1021 tcctgcccca gctcagcatg gtcaagtaca actgcaacaa gtgcaatttc gtcctgggtc 1081 ctttctgcca gtcccagaac caggaggtga aaccaggctc ctgtcctgag tgccagtcgg 1141 ccggcccctt tgaggtcaac atggaggaga ccatctatca gaactaccag cgtatccgaa 1201 tccaggagag tccaggcaaa gtggcggctg gccggctgcc ccgctccaag gacgccattc 1261 tcctcgcaga tctggtggac agctgcaagc caggagacga gatagagctg actggcatct 1321 atcacaacaa ctatgatggc tccctcaaca ctgccaatgg cttccctgtc tttgccactg 1381 tcatcctagc caaccacgtg gccaagaagg acaacaaggt tgctgtaggg gaactgaccg 1441 atgaagatgt gaagatgatc actagcctct ccaaggatca gcagatcgga gagaagatct 1501 ttgccagcat tgctccttcc atctatggtc atgaagacat caagagaggc ctggctctgg 1561 ccctgttcgg aggggagccc aaaaacccag gtggcaagca caaggtacgt ggtgatatca 1621 acgtgctctt gtgcggagac cctggcacag cgaagtcgca gtttctcaag tatattgaga 1681 aagtgtccag ccgagccatc ttcaccactg gccagggggc gtcggctgtg ggcctcacgg 1741 cgtatgtcca gcggcaccct gtcagcaggg agtggacctt ggaggctggg gccctggttc 1801 tggctgaccg aggagtgtgt ctcattgatg aatttgacaa gatgaatgac caggacagaa 1861 ccagcatcca tgaggccatg gagcaacaga gcatctccat ctcgaaggct ggcatcgtca 1921 cctccctgca ggctcgctgc acggtcattg ctgccgccaa ccccatagga gggcgctacg 1981 acccctcgct gactttctct gagaacgtgg acctcacaga gcccatcatc tcacgctttg 2041 acatcctgtg tgtggtgagg gacaccgtgg acccagtcca ggacgagatg ctggcccgct 2101 tcgtggtggg cagccacgtc agacaccacc ccagcaacaa ggaggaggag gggctggcca 2161 atggcagcgc tgctgagccc gccatgccca acacgtatgg cgtggagccc ctgccccagg 2221 aggtcctgaa gaagtacatc atctacgcca aggagagggt ccacccgaag ctcaaccaga 2281 tggaccagga caaggtggcc aagatgtaca gtgacctgag gaaagaatct atggcgacag 2341 gcagcatccc cattacggtg cggcacatcg agtccatgat ccgcatggcg gaggcccacg 2401 cgcgcatcca tcTGCGGGAC TATGTGATCG AAGACGACGT CAACATGGCC ATCCGCGTGA 2461 TGCTGGAGAG CTTCATAGAC ACACAGAAGT TCAGCGTCAT GCGCAGCATG CGCAAGACTT 2521 TTGCCCGCTA CCTTTCATTC CGGCGTGACA ACAATGAGCT GTTGCTCTTC ATACTGAAGC 2581 AGTTAGTGGC AGAGCAGGTG ACATATCAGC GCAACCGCTT TGGGGCCCAG CAGGACACTA 2641 TTGAGGTCCC TGAGAAGGAC TTGGTGGATA AGGCTCGTCA GATCAACATC CACAACCTCT 2701 CTGCATTTTA TGACAGTGAG CTCTTCAGGA TGAACAAGTT CAGCCACGAC CTGAAAAGGA 2761 AAATGATCCT GCAGCAGTTC TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2821 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2881 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT ATTGACCAAT 2941 CTTAGATATT TGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 3001 att