Construct: ORF TRCN0000489324
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020501.1_s317c1
- DNA Barcode:
- TCATGAAGCAAACAGCCCCGAGTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- UHMK1 (127933)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489324
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127933 | UHMK1 | U2AF homology motif kinase 1 | NM_144624.2 | 100% | 100% | |
| 2 | human | 127933 | UHMK1 | U2AF homology motif kinase 1 | NM_175866.5 | 81.9% | 81.3% | (many diffs) |
| 3 | human | 127933 | UHMK1 | U2AF homology motif kinase 1 | NM_001184763.1 | 62.8% | 60.6% | (many diffs) |
| 4 | mouse | 16589 | Uhmk1 | U2AF homology motif (UHM) k... | XM_006496682.3 | 92.7% | 98.8% | (many diffs) |
| 5 | mouse | 16589 | Uhmk1 | U2AF homology motif (UHM) k... | NM_010633.3 | 76% | 80.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1104
- ORF length:
- 1032
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcggga tccggctgcg cctggggcgc ggagccgccg cgttttctgg 121 aggccttcgg gcggctgtgg caggtacaga gccgtctggg tagcggctcc tccgcctcgg 181 tgtatcgggt tcgctgctgc ggcaaccctg gctcgccccc cggcgccctc aagcagttct 241 tgccgccagg aaccaccggg gctgcggcct ctgccgccga gtatggtttc cgcaaagaga 301 gggcggcgct ggaacagttg cagggtcaca gaaacatcgt gactttgtat ggagtgttta 361 caatccactt ttctccaaat gtgccatcac gctgtctgtt gcttgaactc ctggatgtca 421 gtgtttcgga attgctctta tattccagtc accagggttg ttccatgtgg atgatacagc 481 attgtgcccg agatgttttg gaggcccttg cttttcttca tcatgagggc tatgtccatg 541 cggacctcaa accacgtaac atattgtgga gtgcagagaa tgaatgtttt aaactcattg 601 actttggact tagcttcaaa gaaggcaatc aggatgtaaa gtatattcag acagacgggt 661 atcgggctcc agaagcagaa ttgcaaaatt gcttggccca ggctggcctg cagagtgata 721 cagaatgtac ctcagctgtt gatctgtgga gcctaggaat cattttactG GAAATGTTCT 781 CAGGAATGAA ACTGAAACAT ACAGTCAGAT CTCAGGAATG GAAGGCAAAC AGTTCTGCTA 841 TTATTGATCA CATATTTGCC AGTAAAGCAG TGGTGAATGC CGCAATTCCA GCCTATCACC 901 TAAGAGACCT TATCAAAAGC ATGCTTCATG ATGATCCAAG CAGAAGAATT CCTGCTGAAA 961 TGGCATTGTG CAGCCCATTC TTTAGCATTC CTTTTGCCCC TCATATTGAA GATCTGGTCA 1021 TGCTTCCCAC TCCAGTGCTA AGACTGCTGA ATGTGCTGGA TGATGATTAT CTTGAGAATG 1081 AAGAGGAATA TGAAGGTCTT TGTTAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1141 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1201 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCATGAAG 1261 CAAACAGCCC CGAGTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1321 aagatt