Construct: ORF TRCN0000489325
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020279.1_s317c1
- DNA Barcode:
- TTGACCTTTTAAGCTCGAACTATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PDK4 (5166)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489325
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5166 | PDK4 | pyruvate dehydrogenase kina... | NM_002612.4 | 99.6% | 99.7% | 1023A>C;1233_1234insTTG |
| 2 | mouse | 27273 | Pdk4 | pyruvate dehydrogenase kina... | NM_013743.2 | 86.3% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1308
- ORF length:
- 1236
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaaggcg gcccgcttcg tgctgcgcag cgctggctcg ctcaacggcg 121 ccggcctggt gccccgagag gtggagcatt tctcgcgcta cagcccgtcc ccgctgtcca 181 tgaagcagct actggacttt ggttcagaaa atgcatgtga aagaacttct tttgcatttt 241 tgcgacaaga attgcctgtg agactcgcca acattctgaa ggaaattgat atcctcccga 301 cccaattagt aaatacctct tcagtgcaat tggttaaaag ctggtatata cagagcctga 361 tggatttggt ggaattccat gagaaaagcc cagatgacca gaaagcatta tcagactttg 421 tagatacact catcaaagtt cgaaatagac accataatgt agtccctaca atggcacaag 481 gaatcataga gtataaagat gcctgtacag ttgacccagt caccaatcaa aatcttcaat 541 atttcttgga tcgattttac atgaaccgta tttctactcg gatgctgatg aaccagcaca 601 ttcttatatt tagtgactca cagacaggaa acccaagcca cattggaagc attgatccta 661 actgtgatgt ggtagcagtg gtccaagatg cctttgagtg ttcaaggatg ctctgtgatc 721 agtattattt atcatctcca gaattaaagc ttacacaagt gaatggaaaa tttccagacc 781 aaccaattca catcgtgtat gttccttctc acctccatca tatgctcttt gaactattta 841 agaatgcaat gcgggcaaca gttgaacacc aggaaaatca gccttccctt acaccaatag 901 aggttattgt tgtcttggga aaagaagacc ttaccattaa gatttcagac agaggaggtg 961 gtgttccccT GAGAATTATT GACCGCCTCT TTAGTTATAC ATACTCCACT GCACCAACGC 1021 CTGTGATGGA TAATTCCCGG AATGCTCCTT TGGCTGGTTT TGGTTACGGC TTGCCAATTT 1081 CTCGTCTGTA TGCCAAGTAC TTTCAAGGAG ATCTGAATCT CTACTCTTTA TCAGGATATG 1141 GAACAGATGC TATCATCTAC TTAAAGGCTT TGTCTTCTGA GTCTATAGAA AAACTTCCAG 1201 TTTTTAACAA GTCAGCCTTC AAACATTATC AGATGAGCTC TGAGGCTGAT GACTGGTGTA 1261 TCCCAAGCAG GGAACCAAAG AACCTGGCAA AAGAAGTGGC CATGTTGTGA GACCCAGCTT 1321 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1381 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1441 CTTGTGGAAA GGACGATTGA CCTTTTAAGC TCGAACTATC ACGCGTTAAG TCgacaatca 1501 acctctggat tacaaaattt gtgaaagatt