Construct: ORF TRCN0000489364
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019274.1_s317c1
- DNA Barcode:
- GTTCGCCCGGACGTTTAATTCCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADGRD2 (347088)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489364
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 347088 | ADGRD2 | adhesion G protein-coupled ... | XM_006717348.3 | 80.9% | 98.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 2964
- ORF length:
- 2889
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggac gccccctggg gagccggcga aagatggctg catggcgccg 121 ctgtggacag atccggcgtg tcactgggcc ctccacccac ccctcaggtc aaccagggca 181 cactgggccc acaggtcgca cctgtcgccg caggcgaggt ggtcaaaaca gccggcggag 241 tgtgcaagtt cagcggccag cggctgagct ggtggcaggc tcaggaaagc tgcgagcagc 301 agttcggcca cctggccctg cagcctccag atggcgtgct cgccagcaga ctgagggacc 361 ctgtgtgggt cggacagaga gaggcccccc tgagaaggcc tccccagcgg agagccagaa 421 ccaccgccgt gctggtgttc gacgagcgga ccgccgacag agccgccaga ctgagaagcc 481 ctctgcctga gctggccgcc ctgaccgcct gtacccacgt gcagtgggac tgcgcttccc 541 ctgatcctgc cgccctgttt tctgtggctg cccctgccct gcccaacgcc ctgcagctga 601 gagcctttgc cgagcctggc ggagtcgtca gagccgctct ggtcgtcaga ggacagcatg 661 ccccctttct ggccgccttt cgggccgatg gccggtggca tcacgtgtgc gccacatggg 721 agcagagggg cggcagatgg gccctgttta gcgacggcag acggcgggct ggcgccagag 781 gactgggagc tggacaccct gtgccctctg gcggcattct cgtgctgggc caggaccagg 841 atagcctggg cggaggcttt agcgtccggc acgccctgag cggcaacctg accgacttcc 901 acctgtgggc tcgcgccctg agccctgccc agctgcacag agccagagcc tgtgcccctc 961 ctagcgaggg cctgctgttc cgctgggacc ctggcgccct ggacgtgacc cctagcctgc 1021 tgcctaccgt gtgggtccga ctgctgtgcc ccgtgcccag cgaggaatgc cccacctgga 1081 accctggccc cagaagcgag ggcagcgagc tgtgcctgga accccagccc ttcctgtgct 1141 gctaccggac cgagccctac agacggctgc aggatgccca gtcttggcct ggacaggacg 1201 tgatcagcag agtgaacgcc ctggccaacg acatcgtgct gctgcccgac cccctgtctg 1261 aagtgcacgg cgccctgtct ccagccgagg ccagctcttt cctgggcctg ctggaacatg 1321 tgctggccat ggaaatggcc cccctgggac ctgccgcact gctggccgtc gtgcggttcc 1381 tgaagagagt ggtcgctctg ggcgcaggcg atcctgaact gctgctgaca ggcccctggg 1441 agcagctgag ccagggcgtg gtgtctgtgg cctctctggt gctggaagaa caggtggccg 1501 acacctggct gtctctgcgg gaagtgatcg gcggacctat ggccctggtg gccagcgtgc 1561 agagactggc ccctctgctg agcaccagca tgaccagcga gcggcccaga atgcggatcc 1621 agcacagaca cgccggcctg agcggcgtga ccgtgatcca cagctggttc accagccggg 1681 tgttccagca caccctggaa ggccccgatc tggaacctca ggctccagcc agcagcgaag 1741 aggccaaccg ggtgcagaga ttcctgagca cccaggtcgg aagcgccatc atcagctccg 1801 aagtgtggga cgtgaccggc gaagtgaacg tggccatgac attccatctg cagcaccggg 1861 cccagagccc cctgtttcct ccacaccccc ccagcccata cacaggcgga gcctgggcta 1921 ccactggctg tagcgtcgcc gctctgtacc tggacagcac cgcctgtttc tgcaaccaca 1981 gcaccagctt cgccatcctg ctgcagatct acgaggtgca gagaggccct gaggaagaga 2041 gcctgctgcg gaccctgagc ttcgtgggct gcggcgtgtc cttctgcgcc ctgacaacca 2101 ccttcctgct gtttctggtg gccggggtgc ccaagagcga gagaaccacc gtgcacaaga 2161 acctgacctt cagcctggcc agcgccgagg gcttcctgat gacctctgag tgggccaagg 2221 ccaacgaggt ggcctgtgtg gccgtgaccg tggctatgca cttcctgttc ctcgtggcct 2281 tcagctggat gctggtggaa ggcctgctgc tgtggcggaa ggtggtggcc gtgtccatgc 2341 accccggacc cggcatgaga ctgtaccacg ccacaggatg gggcgtgcca gtgggcatcg 2401 tggctgtgac cctggccatg ctgccccacg attacgtggc cccaggccac tgctggctga 2461 acgtgcacac caacgccatc tgggccttcg tgggccccgt gctgttcgtg ctgaccgcca 2521 atacctgcat cctggcccgg gtggtcatga tcaccgtgtc cagcgctcgc cggagagccc 2581 ggatgctgag ccctcagcct tgccTGCAGC AGCAGATCTG GACACAGATT TGGGCCACCG 2641 TGAAGCCTGT GCTGGTCCTG CTGCCCGTGC TGGGACTGAC ATGGCTGGCC GGAATTCTGG 2701 TGCACCTGAG TCCAGCCTGG GCCTATGCCG CCGTGGGCCT GAATAGCATC CAGGGCCTGT 2761 ACATCTTCCT GGTGTACGCC GCCTGCAACG AGGAAGTGCG GAGCGCCCTG CAGAGAATGG 2821 CCGAGAAGAA AGTCGCCGAG GTGCTGAGGG CTCTGGGAGT GTGGGGCGGA GCCGCCAAAG 2881 AGCACAGCCT GCCCTTCAGC GTGCTGCCCC TGTTCCTGCC TCCCAAGCCC AGCACCCCCA 2941 GACACCCTCT GAAGGCTCCC GCCGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 3001 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 3061 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG TTCGCCCGGA 3121 CGTTTAATTC CTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 3181 att