Construct: ORF TRCN0000489432
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021109.1_s317c1
- DNA Barcode:
- AAGACGCCGAAACGTAATCGCATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R3 (50831)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489432
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50831 | TAS2R3 | taste 2 receptor member 3 | NM_016943.2 | 99.8% | 100% | 807C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1020
- ORF length:
- 948
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgatggga ctcaccgagg gggtgttcct gattctgtct ggcactcagt 121 tcacactggg aattctggtc aattgtttca ttgagttggt caatggtagc agctggttca 181 agaccaagag aatgtctttg tctgacttca tcatcaccac cctggcactc ttgaggatca 241 ttctgctgtg tattatcttg actgatagtt ttttaataga attctctccc aacacacatg 301 attcagggat aataatgcaa attattgatg tttcctggac atttacaaac catctgagca 361 tttggcttgc cacctgtctt ggtgtcctct actgcctgaa aatcgccagt ttctctcacc 421 ccacattcct ctggctcaag tggagagttt ctagggtgat ggtatggatg ctgttgggtg 481 cactgctctt atcctgtggt agtaccgcat ctctgatcaa tgagtttaag ctctattctg 541 tctttagggg aattgaggcc accaggaatg tgactgaaca cttcagaaag aagaggagtg 601 agtattatct gatccatgtt cttgggactc tgtggtacct gcctccctta attgtgtccc 661 tggcctccta ctctttgctc aTCTTCTCCC TGGGGAGGCA CACACGGCAG ATGCTGCAAA 721 ATGGGACAAG CTCCAGAGAT CCAACCACTG AGGCCCACAA GAGGGCCATC AGAATCATCC 781 TTTCCTTCTT CTTTCTCTTC TTACTTTACT TTCTTGCTTT CTTAATTGCA TCATTTGGTA 841 ATTTCCTACC AAAAACCAAG ATGGCTAAGA TGATTGGTGA AGTAATGACA ATGTTTTATC 901 CTGCTGGCCA CTCATTTATT CTCATTCTGG GGAACAGTAA GCTGAAGCAG ACATTTGTAG 961 TGATGCTCCG GTGTGAGTCT GGTCATCTGA AGCCTGGATC CAAGGGACCC ATTTTCTCTT 1021 AGGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1081 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1141 GGCTTTATAT ATCTTGTGGA AAGGACGAAA GACGCCGAAA CGTAATCGCA TCACGCGTTA 1201 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt