Construct: ORF TRCN0000489466
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020581.1_s317c1
- DNA Barcode:
- TCGACTAGCATCCTCTAGGTACCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NEK8 (284086)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489466
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284086 | NEK8 | NIMA related kinase 8 | NM_178170.3 | 100% | 100% | |
| 2 | mouse | 140859 | Nek8 | NIMA (never in mitosis gene... | NM_080849.3 | 86.9% | 93.4% | (many diffs) |
| 3 | mouse | 140859 | Nek8 | NIMA (never in mitosis gene... | XM_006532184.3 | 86.8% | 93.1% | (many diffs) |
| 4 | mouse | 140859 | Nek8 | NIMA (never in mitosis gene... | XM_006532183.3 | 85.6% | 91.8% | (many diffs) |
| 5 | mouse | 140859 | Nek8 | NIMA (never in mitosis gene... | XM_006532185.3 | 79.9% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2148
- ORF length:
- 2076
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagaag tacgagcgga tccgagtggt ggggagaggt gccttcggga 121 ttgtgcacct gtgcctgcga aaggctgacc agaagctggt gatcatcaag cagattccag 181 tggaacagat gaccaaggaa gagcggcagg cagcccagaa tgagtgccag gtcctcaagc 241 tgctcaacca ccccaatgtc attgagtact acgagaactt cctggaagac aaagccctta 301 tgatcgccat ggaatatgca ccaggcggca ctctggctga gttcatccaa aagcgctgta 361 attccctgct ggaggaggag accatcctgc acttcttcgt gcagatcctg cttgcactgc 421 atcatgtgca cacccacctc atcctgcacc gagacctcaa gacccagaac atcctgcttg 481 acaaacaccg catggtcgtc aagatcggtg atttcggcat ctccaagatc cttagcagca 541 agagcaaggc ctacacggtg gtgggtaccc catgctatat ctcccctgag ctgtgtgagg 601 gcaagcccta caaccagaag agtgacatct gggccctggg ctgtgtcctc tacgagctgg 661 ccagcctcaa gagggctttc gaggctgcga acttgccagc actggtgctg aagatcatga 721 gtggcacctt tgcacctatc tctgaccggt acagccctga gcttcgccag ctggtcctga 781 gtctactcag cctggagcct gcccagcggc caccactcag ccacatcatg gcacagcccc 841 tctgcatccg tgccctcctc aacctccaca ccgacgtggg cagtgtccgc atgcggaggg 901 cagagaagtc cgtggccccc agcaacacag ggagcaggac caccagtgtc cgctgcagag 961 gtatcccccg gggacctgtg aggccagcca tcccaccacc actgtcgtca gtgtatgcct 1021 ggggtggtgg gctgggcacc cccctgcggc tgccaatgct caacacagag gtggtccagg 1081 tggcagctgg gcgcacgcag aaagccggcg tcacgcgctc tgggcgtctc atcctgtggg 1141 aggccccacc cctaggtgca ggcggaggca gtctccttcc tggggcagtg gagcagccac 1201 agccccagtt catctcgcgt ttcctggagg gccagtcggg tgtgaccatc aagcacgtgg 1261 cctgtgggga cttcttcact gcctgcctga ctgacagagg catcatcatg acattcggca 1321 gcggcagcaa tgggtgccta ggccatggca gcctcactga catcagccag cccaccattg 1381 tggaggcttt gctgggctat gaaatggtgc aggtggcctg tggggcctct cacgtgctgg 1441 ccctgtccac tgagcgagaa ctatttgcct ggggccgtgg agacagcggc agactggggc 1501 taggcaccag ggagtcccac agctgccccc agcaggtgcc catgccccca ggacaggaag 1561 ctcagcgagt tgtatgtggt atcgattcct ccatgatcct cactgtgcct ggccaagccc 1621 tagcctgtgg gagcaacagg ttcaacaagc tgggcctgga ccacctctcc ctgggggagg 1681 agcctgtccc ccaccagcaa gtggaggagg ccctgagctt cacactacta ggctctgcac 1741 ccctggacca ggagcctctg ctgagtatag acctgggcac tgctcacTCA GCTGCTGTGA 1801 CTGCCTCGGG TGATTGCTAC ACTTTTGGCA GCAATCAGCA CGGACAGTTG GGCACCAATA 1861 CTCGCCGAGG CAGTCGGGCA CCCTGTAAGG TCCAAGGCCT TGAGGGCATC AAGATGGCAA 1921 TGGTAGCCTG TGGGGATGCC TTCACTGTAG CTATTGGGGC AGAGAGCGAA GTGTACTCTT 1981 GGGGCAAAGG GGCGCGAGGT CGATTGGGAA GGAGGGATGA GGATGCCGGA CTCCCTCGGC 2041 CAGTGCAGTT GGATGAGACA CACCCTTACA CGGTGACTTC CGTGTCCTGT TGCCATGGAA 2101 ACACCCTCCT GGCTGTTCGA TCGGTCACAG ATGAGCCGGT CCCCCCCTAG AACCCAGCTT 2161 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2221 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2281 CTTGTGGAAA GGACGATCGA CTAGCATCCT CTAGGTACCT ACGCGTTAAG TCgacaatca 2341 acctctggat tacaaaattt gtgaaagatt