Construct: ORF TRCN0000489468
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020371.1_s317c1
- DNA Barcode:
- ATGTCCTGTAGTCCGTTCGCAGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CLK3 (1198)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489468
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1198 | CLK3 | CDC like kinase 3 | NM_003992.4 | 100% | 100% | |
| 2 | human | 1198 | CLK3 | CDC like kinase 3 | NM_001130028.1 | 76.8% | 76.8% | 1_444del |
| 3 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_006510739.3 | 92.7% | 98.7% | (many diffs) |
| 4 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313060.1 | 92.7% | 98.7% | (many diffs) |
| 5 | mouse | 102414 | Clk3 | CDC-like kinase 3 | NM_007713.4 | 71.2% | 75.8% | (many diffs) |
| 6 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313061.1 | 51.7% | 54.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1542
- ORF length:
- 1470
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcatcac tgtaagcgat accgctcccc tgaaccagac ccgtacctga 121 gctaccgatg gaagaggagg aggtcctaca gtcgggaaca tgaagggaga ctgcgatacc 181 cgtcccgaag ggagcctccc ccacgaagat ctcggtccag aagccatgac cgcctgccct 241 accagaggag gtaccgggag cgccgtgaca gcgatacata ccggtgtgaa gagcggagcc 301 catcctttgg agaggactac tatggacctt cacgttctcg tcatcgtcgg cgatcgcggg 361 agagggggcc ataccggacc cgcaagcatg cccaccactg ccacaaacgc cgcaccaggt 421 cttgtagcag cgcctcctcg agaagccaac agagcagtaa gcgcagcagc cggagtgtgg 481 aagatgacaa ggagggtcac ctggtgtgcc ggatcggcga ttggctccaa gagcgatatg 541 agattgtggg gaacctgggt gaaggcacct ttggcaaggt ggtggagtgc ttggaccatg 601 ccagagggaa gtctcaggtt gccctgaaga tcatccgcaa cgtgggcaag taccgggagg 661 ctgcccggct agaaatcaac gtgctcaaaa aaatcaagga gaaggacaaa gaaaacaagt 721 tcctgtgtgt cttgatgtct gactggttca acttccacgg tcacatgtgc atcgcctttg 781 agctcctggg caagaacacc tttgagttcc tgaaggagaa taacttccag ccttaccccc 841 taccacatgt ccggcacatg gcctaccagc tctgccacgc ccttagattt ctgcatgaga 901 atcagctgac ccatacagac ttgaaaccag agaacatcct gtttgtgaat tctgagtttg 961 aaaccctcta caatgagcac aagagctgtg aggagaagtc agtgaagaac accagcatcc 1021 gagtggctga ctttggcagt gccacatttg accatgagca ccacaccacc attgtggcca 1081 cccgtcacta tcgcccgcct gaggtgatcc ttgagctggg ctgggcacag cccTGTGACG 1141 TCTGGAGCAT TGGCTGCATT CTCTTTGAGT ACTACCGGGG CTTCACACTC TTCCAGACCC 1201 ACGAAAACCG AGAGCACCTG GTGATGATGG AGAAGATCCT AGGGCCCATC CCATCACACA 1261 TGATCCACCG TACCAGGAAG CAGAAATATT TCTACAAAGG GGGCCTAGTT TGGGATGAGA 1321 ACAGCTCTGA CGGCCGGTAT GTGAAGGAGA ACTGCAAACC TCTGAAGAGT TACATGCTCC 1381 AAGACTCCCT GGAGCACGTG CAGCTGTTTG ACCTGATGAG GAGGATGTTA GAATTTGACC 1441 CTGCCCAGCG CATCACACTG GCCGAGGCCC TGCTGCACCC CTTCTTTGCT GGCCTGACCC 1501 CTGAGGAGCG GTCCTTCCAC ACCAGCCGCA ACCCAAGCAG ATAGGACCCA GCTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA ATGTCCTGTA GTCCGTTCGC AGTCACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt