Construct: ORF TRCN0000489470
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020233.1_s317c1
- DNA Barcode:
- GAATTGAAAAGGTTGTGAACATGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STK10 (6793)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489470
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6793 | STK10 | serine/threonine kinase 10 | NM_005990.4 | 99.8% | 100% | 756G>A;783T>C;2619T>C |
| 2 | human | 6793 | STK10 | serine/threonine kinase 10 | XM_017009788.1 | 88.7% | 88.8% | (many diffs) |
| 3 | mouse | 20868 | Stk10 | serine/threonine kinase 10 | NM_009288.2 | 86% | 88.5% | (many diffs) |
| 4 | mouse | 20868 | Stk10 | serine/threonine kinase 10 | XM_017314404.1 | 67.8% | 68.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2976
- ORF length:
- 2904
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctttt gccaatttcc gccgcatcct gcgcctgtct accttcgaga 121 agagaaagtc ccgcgaatat gagcacgtcc gccgcgacct ggaccccaac gaggtgtggg 181 agatcgtggg cgagctgggc gacggcgcct tcggcaaggt ttacaaggcc aagaataagg 241 agacgggtgc tttggctgcg gccaaagtca ttgaaaccaa gagtgaggag gagctggagg 301 actacatcgt ggagattgag atcctggcca cctgcgacca cccctacatt gtgaagctcc 361 tgggagccta ctatcacgac gggaagctgt ggatcatgat tgagttctgt ccagggggag 421 ccgtggacgc catcatgctg gagctggaca gaggcctcac ggagccccag atacaggtgg 481 tttgccgcca gatgctagaa gccctcaact tcctgcacag caagaggatc atccaccgag 541 atctgaaagc tggcaacgtg ctgatgaccc tcgagggaga catcaggctg gctgactttg 601 gtgtgtctgc caagaatctg aagactctac agaaacgaga ttccttcatc ggcacgcctt 661 actggatggc ccccgaggtg gtcatgtgtg agaccatgaa agacacgccc tacgactaca 721 aagccgacat ctggtccctg ggcatcacgc tgattgagat ggcccagatc gagccgccac 781 accacgagct caaccccatg cgggtcctgc taaagatcgc caagtcagac cctcccacgc 841 tgctcacgcc ctccaagtgg tctgtagagt tccgtgactt cctgaagata gccctggata 901 agaacccaga aacccgaccc agtgccgcgc agctgctgga gcatcccttc gtcagcagca 961 tcaccagtaa caaggctctg cgggagctgg tggctgaggc caaggccgag gtgatggaag 1021 agatcgaaga cggccgggat gagggggaag aggaggacgc cgtggatgcc gcctccaccc 1081 tggagaacca tactcagaac tcctctgagg tgagtccgcc aagcctcaat gctgacaagc 1141 ctctcgagga gtcaccttcc accccgctgg cacccagcca gtctcaggac agtgtgaatg 1201 agccctgcag ccagccctct ggggacagat ccctccaaac caccagtccc ccagtcgtgg 1261 cccctggaaa tgagaacggc ctggcagtgc ctgtgcccct gcggaagtcc cgacccgtgt 1321 caatggatgc cagaattcag gtagcccagg agaagcaagt tgctgagcag ggtggggacc 1381 tcagcccagc agccaacaga tctcaaaagg ccagccagag ccggcccaac agcagcgccc 1441 tggagacctt gggtggggag aagctggcca atggcagcct ggagccacct gcccaggcag 1501 ctccagggcc ttccaagagg gactcggact gcagcagcct ctgcacctct gagagcatgg 1561 actatggtac caatctctcc actgacctgt cgctgaacaa agagatgggc tctctgtcca 1621 tcaaggaccc gaaactgtac aaaaaaaccc tcaagcggac acgcaaattt gtggtggatg 1681 gtgtggaggt gagcatcacc acctccaaga tcatcagcga agatgagaag aaggatgagg 1741 agatgagatt tctcaggcgc caggaactcc gagagcttcg gctgctccag aaagaagagc 1801 atcggaacca gacccagctg agtaacaagc atgagctgca gctggagcaa atgcataaac 1861 gttttgaaca ggaaatcaac gccaagaaga agttctttga cacggaatta gagaacctgg 1921 agcgtcagca aaagcagcaa gtggagaaga tggagcaaga ccatgccgtg cgccgccggg 1981 aggaggccag gcggatccgc ctggagcagg atcgggacta caccaggttc caagagcagc 2041 tcaaactgat gaagaaagag gtgaagaacg aggtggagaa gctcccccga cagcagcgga 2101 aggaaagcat gaagcagaag atggaggagc acacgcagaa aaagcagctt cttgaccggg 2161 actttgtagc caagcagaag gaggacctgg agctggccat gaagaggctc accaccgaca 2221 acaggcggga gatctgtgac aaggagcgcg agtgcctcat gaagaagcag gagctccttc 2281 gagaccggga agcagccctg tgggagatgg aagagcacca gctgcaggag aggcaccagc 2341 tggtgaagca gcagctcaaa gaccagtact tcctccagcg gcacgagctg ctgcgcaagc 2401 atgagaagga gcgggagcag atgcagcgct acaaccagcg catgatagag cagctgaagg 2461 tgcggcagca acaggaaaag gcgcggctgc ccaagatcca gaggagtgag ggcaagacgc 2521 gcatggccat gtacaagaag agccTCCACA TCAACGGCGG GGGCAGCGCA GCTGAGCAGC 2581 GTGAGAAGAT CAAGCAGTTC TCCCAGCAGG AGGAGAAGAG GCAGAAGTCG GAGCGGCTGC 2641 AGCAACAGCA GAAACACGAG AACCAGATGC GGGACATGCT GGCGCAGTGC GAGAGCAACA 2701 TGAGCGAGCT GCAGCAGCTG CAGAATGAAA AGTGCCACCT CCTGGTAGAG CACGAAACCC 2761 AGAAACTGAA GGCCCTGGAT GAGAGCCATA ACCAGAACCT GAAGGAATGG CGGGACAAGC 2821 TTCGGCCGCG CAAGAAGGCT CTGGAAGAGG ATCTGAACCA GAAGAAGCGG GAGCAGGAGA 2881 TGTTCTTCAA GCTGAGCGAG GAGGCGGAGT GCCCAAACCC CTCCACCCCA AGCAAGGCCG 2941 CCAAGTTCTT CCCCTACAGT TCTGCGGATG CTTCTTGAGA CCCAGCTTTC TTGTACAAAG 3001 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 3061 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 3121 ACGAGAATTG AAAAGGTTGT GAACATGCAC GCGTTAAGTC gacaatcaac ctctggatta 3181 caaaatttgt gaaagatt