Construct: ORF TRCN0000489472
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019249.1_s317c1
- DNA Barcode:
- CCAACCCCTACGACAGTCGAGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SUCNR1 (56670)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489472
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56670 | SUCNR1 | succinate receptor 1 | NM_033050.6 | 99.9% | 99.7% | 1002_1003insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1080
- ORF length:
- 1005
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgctg gggatcatgg catggaatgc aacttgcaaa aactggctgg 121 cagcagaggc tgccctggaa aagtactacc tttccatttt ttatgggatt gagttcgttg 181 tgggagtcct tggaaatacc attgttgttt acggctacat cttctctctg aagaactgga 241 acagcagtaa tatttatctc tttaacctct ctgtctctga cttagctttt ctgtgcaccc 301 tccccatgct gataaggagt tatgccaatg gaaactggat atatggagac gtgctctgca 361 taagcaaccg atatgtgctt catgccaacc tctataccag cattctcttt ctcactttta 421 tcagcataga tcgatacttg ataattaagt atcctttccg agaacacctt ctgcaaaaga 481 aagagtttgc tattttaatc tccttggcca tttgggtttt agtaacctta gagttactac 541 ccatacttcc ccttataaat cctgttataa ctgacaatgg caccacctgt aatgattttg 601 caagttctgg agaccccaac tacaacctca tttacagcat gtgtctaaca ctgttggggt 661 tccttattcc tctttttgtg atgtgtttct tttattacaa gattgctctc ttcctaaagc 721 agaggaatag gcaggttGCT ACTGCTCTGC CCCTTGAAAA GCCTCTCAAC TTGGTCATCA 781 TGGCAGTGGT AATCTTCTCT GTGCTTTTTA CACCCTATCA CGTCATGCGG AATGTGAGGA 841 TCGCTTCACG CCTGGGGAGT TGGAAGCAGT ATCAGTGCAC TCAGGTCGTC ATCAACTCCT 901 TTTACATTGT GACACGGCCT TTGGCCTTTC TGAACAGTGT CATCAACCCT GTCTTCTATT 961 TTCTTTTGGG AGATCACTTC AGGGACATGC TGATGAATCA ACTGAGACAC AACTTCAAAT 1021 CCCTTACATC CTTTAGCAGA TGGGCTCATG AACTCCTACT TTCATTCAGA GAAAAGGACC 1081 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1141 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1201 ATATATCTTG TGGAAAGGAC GACCAACCCC TACGACAGTC GAGTGCACGC GTTAAGTCga 1261 caatcaacct ctggattaca aaatttgtga aagatt