Construct: ORF TRCN0000489508
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019470.1_s317c1
- DNA Barcode:
- CTTACGAAATTTCGTCTAAATGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRM5 (1133)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489508
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1133 | CHRM5 | cholinergic receptor muscar... | NM_001320917.2 | 99.9% | 99.8% | 1596_1597insG |
| 2 | human | 1133 | CHRM5 | cholinergic receptor muscar... | NM_012125.4 | 99.9% | 99.8% | 1596_1597insG |
| 3 | mouse | 213788 | Chrm5 | cholinergic receptor, musca... | NM_205783.2 | 88.1% | 88.5% | (many diffs) |
| 4 | mouse | 213788 | Chrm5 | cholinergic receptor, musca... | XM_006499099.3 | 88.1% | 88.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1674
- ORF length:
- 1599
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggaa ggggattctt accacaatgc aaccaccgtc aatggcaccc 121 cagtaaatca ccagcctttg gaacgccaca ggttgtggga agtcatcacc attgcagctg 181 tgactgctgt ggtaagcctg atcaccattg tgggcaatgt cttggtcatg atctccttca 241 aagtcaacag ccagctcaag acagttaaca actattacct gctcagctta gcctgtgcag 301 atctcatcat tggaatcttc tccatgaacc tctacaccac ctacatcctc atgggacgct 361 gggctctcgg gagtctggct tgtgaccttt ggcttgcact ggactacgtg gccagcaacg 421 cttctgtcat gaaccttctg gtgatcagtt ttgaccgtta cttttccatc acaagaccct 481 tgacatatcg ggccaagcgt actccgaaaa gggctggcat catgattggc ttggcctggc 541 tgatctcctt catcctctgg gccccagcaa tcctctgctg gcagtacttg gttgggaagc 601 ggacagttcc actggatgag tgccagatcc agtttctctc tgagcccacc atcacttttg 661 gcactgccat tgctgccttc tacatccctg tttctgtcat gaccatcctc tactgtcgaa 721 tctaccggga aacagagaag cgaaccaagg acctggctga cctccagggt tctgactctg 781 tgaccaaagc tgagaagaga aagccagctc atagggctct gttcagatcc tgcttgcgct 841 gtcctcgacc caccctggcc cagcgggaaa ggaaccaggc ctcctggtca tcctcccgca 901 ggagcacctc caccactggg aagccatccc aagccactgg cccaagcgcc aattgggcca 961 aagctgagca gctcaccacc tgtagcagct acccttcctc agaggatgag gacaagcccg 1021 ccactgaccc tgtcctccaa gtggtctaca agagtcaggg taaggaaagc ccaggggaag 1081 aattcagtgc tgaagagact gaggaaactt ttgtgaaagc tgaaactgaa aaaagtgact 1141 atgacacccc aaactacctt ctgtctccag cagctgctca tagacccaag agtcagaaat 1201 gtgtggccta taagttccga ttggtggtaa aagctgacgg gaaccaggag accaacaatg 1261 gctgtcacaa ggtgaaaatc atgccctgcc ccttcccagt ggccaaggaa ccttcaacga 1321 aaggcctcaa tcccaacccc agccatcaaa tgaccaaacg aaagagagtg gTCCTAGTCA 1381 AAGAGAGGAA AGCAGCCCAG ACACTGAGTG CCATTCTCCT GGCCTTCATC ATCACATGGA 1441 CCCCGTATAA CATCATGGTC CTGGTTTCTA CCTTCTGTGA CAAGTGTGTC CCAGTCACCC 1501 TGTGGCACTT GGGCTATTGG TTGTGCTATG TCAATAGCAC TGTCAACCCC ATCTGCTATG 1561 CCCTCTGCAA CAGAACCTTC AGGAAGACCT TTAAGATGCT GCTTCTCTGC CGATGGAAAA 1621 AGAAAAAAGT GGAAGAGAAG TTGTACTGGC AGGGGAACAG CAAGCTACCC GACCCAGCTT 1681 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1741 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1801 CTTGTGGAAA GGACGACTTA CGAAATTTCG TCTAAATGAA ACGCGTTAAG TCgacaatca 1861 acctctggat tacaaaattt gtgaaagatt