Construct: ORF TRCN0000489521
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021113.1_s317c1
- DNA Barcode:
- GACCTATCAATTGGCACGATGACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R14 (50840)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489521
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50840 | TAS2R14 | taste 2 receptor member 14 | NM_023922.1 | 100% | 100% | |
| 2 | human | 106707243 | PRH1-TAS2R14 | PRH1-TAS2R14 readthrough | NM_001316893.2 | 45.2% | 38.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1023
- ORF length:
- 951
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggtggt gtcataaaga gcatatttac attcgtttta attgtggaat 121 ttataattgg aaatttagga aatagtttca tagcactggt gaactgtatt gactgggtca 181 agggaagaaa gatctcttcg gttgatcgga tcctcactgc tttggcaatc tctcgaatta 241 gcctggtttg gttaatattc ggaagctggt gtgtgtctgt gtttttccca gctttatttg 301 ccactgaaaa aatgttcaga atgcttacta atatctggac agtgatcaat cattttagtg 361 tctggttagc tacaggcctc ggtacttttt attttctcaa gatagccaat ttttctaact 421 ctatttttct ctacctaaag tggagggtta aaaaggtggt tttggtgctg cttcttgtga 481 cttcggtctt cttgttttta aatattgcac tgataaacat ccatataaat gccagtatca 541 atggatacag aagaaacaag acttgcagtt ctgattcaag taactttaca cgattttcca 601 gtcttattGT ATTAACCAGC ACTGTGTTCA TTTTCATACC CTTTACTTTG TCCCTGGCAA 661 TGTTTCTTCT CCTCATCTTC TCCATGTGGA AACATCGCAA GAAGATGCAG CACACTGTCA 721 AAATATCCGG AGACGCCAGC ACCAAAGCCC ACAGAGGAGT TAAAAGTGTG ATCACTTTCT 781 TCCTACTCTA TGCCATTTTC TCTCTGTCTT TTTTCATATC AGTTTGGACC TCTGAAAGGT 841 TGGAGGAAAA TCTAATTATT CTTTCCCAGG TGATGGGAAT GGCTTATCCT TCATGTCACT 901 CATGTGTTCT GATTCTTGGA AACAAGAAGC TGAGACAGGC CTCTCTGTCA GTGCTACTGT 961 GGCTGAGGTA CATGTTCAAA GATGGGGAGC CCTCAGGTCA CAAAGAATTT AGAGAATCAT 1021 CTTGAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1081 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1141 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGACCTATCA ATTGGCACGA TGACCACGCG 1201 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt