Construct: ORF TRCN0000489523
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020793.1_s317c1
- DNA Barcode:
- TAATAAAATGTGTGCTATCTTTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- AGTR2 (186)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489523
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 186 | AGTR2 | angiotensin II receptor type 2 | NM_000686.5 | 99.9% | 100% | 51T>C |
| 2 | human | 186 | AGTR2 | angiotensin II receptor type 2 | XM_011537533.1 | 99.9% | 100% | 51T>C |
| 3 | mouse | 11609 | Agtr2 | angiotensin II receptor, ty... | NM_007429.5 | 88.8% | 91.7% | (many diffs) |
| 4 | mouse | 11609 | Agtr2 | angiotensin II receptor, ty... | XM_006527555.1 | 82.1% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1161
- ORF length:
- 1089
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaagggc aactccaccc ttgccactac tagcaaaaac attaccagcg 121 gccttcactt cgggcttgtg aacatctctg gcaacaatga gtctaccttg aactgttcac 181 agaaaccatc agataagcat ttagatgcaa ttcctattct ttactacatt atatttgtaa 241 ttggatttct ggtcaatatt gtcgtggtta cactgttttg ttgtcaaaag ggtcctaaaa 301 aggtttctag catatacatc ttcaacctcg ctgtggctga tttactcctt ttggctactc 361 ttcctctatg ggcaacctat tattcttata gatatgactg gctctttgga cctgtgatgt 421 gcaaagtttt tggttctttt cttaccctga acatgtttgc aagcattttt tttatcacct 481 gcatgagtgt tgataggtac caatctgtca tctacccctt tctgtctcaa agaagaaatc 541 cctggcaagc atcttatata gttccccttg tttggtgtat ggcctgtttg tcctcattgc 601 caacatttta ttttcgagac gtcagaacca ttgaatactt aggagtgaat gcttgcatta 661 tggctttccc acctgagaaa tatgcccaat ggtcagctgg gattgcctta atgaaaaata 721 tccttggttt tattatccct ttaatattca tagcaacatg ctattttGGA ATTAGAAAAC 781 ACTTACTGAA GACGAATAGC TATGGGAAGA ACAGGATAAC CCGTGACCAA GTCCTGAAGA 841 TGGCAGCTGC TGTTGTTCTG GCCTTCATCA TTTGCTGGCT TCCCTTCCAT GTTCTGACCT 901 TCCTGGATGC TCTGGCCTGG ATGGGTGTCA TTAATAGCTG CGAAGTTATA GCAGTCATTG 961 ACCTGGCACT TCCTTTTGCC ATCCTCTTGG GATTCACCAA CAGCTGCGTT AATCCGTTTC 1021 TGTATTGTTT TGTTGGAAAC CGGTTCCAAC AGAAGCTCCG CAGTGTGTTT AGGGTTCCAA 1081 TTACTTGGCT CCAAGGGAAA AGAGAGAGTA TGTCTTGCCG GAAAAGCAGT TCTCTTAGAG 1141 AAATGGAGAC CTTTGTGTCT TAGGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1201 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1261 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AATAAAATGT 1321 GTGCTATCTT TTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1381 att