Construct: ORF TRCN0000489539
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019392.1_s317c1
- DNA Barcode:
- TTCCGATTTCTTATCCAACCCCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAAR5 (9038)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489539
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9038 | TAAR5 | trace amine associated rece... | NM_003967.2 | 99.9% | 99.7% | 1011_1012insG |
| 2 | mouse | 215854 | Taar5 | trace amine-associated rece... | NM_001009574.1 | 85.3% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1089
- ORF length:
- 1014
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgaga gctgtcttca tccaaggtgc tgaagagcac cctgcggcat 121 tctgctacca ggtgaatggg tcttgcccca ggacagtaca tactctgggc atccagttgg 181 tcatctacct ggcctgtgca gcaggcatgc tgattatcgt gctagggaat gtatttgtgg 241 catttgctgt gtcctacttc aaagcgcttc acacgcccac caacttcctg ctgctctccc 301 tggccctggc tgacatgttt ctgggtctgc tggtgctgcc cctcagcacc attcgctcag 361 tggagagctg ctggttcttc ggggacttcc tctgccgcct gcacacctac ctggacaccc 421 tcttctgcct cacctccatc ttccatctct gtttcatttc cattgaccgc cactgtgcca 481 tctgtgaccc cctgctctat ccctccaagt tcacagtgag ggtggctctc aggtacatcc 541 tggcaggatg gggggtgccc gcagcataca cttcgttatt cctctacaca gatgtggtag 601 agacaaggct cagccagtgg ctggaagaga tgccttgtgt gggcagttgc cagctgctgc 661 tcaataaatt ttggggctgg ttaaacttcc ctttgttctt tgtcccctgc ctcattatga 721 tcagcttgta tgtgaagatc tttgtggttg ctaCCAGACA GGCTCAGCAG ATTACCACAT 781 TGAGCAAAAG CCTGGCTGGG GCTGCCAAGC ATGAGAGAAA AGCTGCCAAG ACCCTGGGCA 841 TTGCTGTGGG CATATACCTC TTGTGCTGGC TGCCCTTCAC CATAGACACG ATGGTCGACA 901 GCCTCCTTCA CTTTATCACA CCCCCACTGG TCTTTGACAT CTTTATCTGG TTTGCTTACT 961 TCAACTCAGC CTGCAACCCC ATCATCTATG TCTTTTCCTA CCAGTGGTTT CGGAAGGCAC 1021 TGAAACTCAC ACTGAGCCAG AAGGTCTTCT CACCGCAGAC ACGCACTGTT GATTTGTACC 1081 AAGAAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1141 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1201 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTCCGATTT CTTATCCAAC CCCGCACGCG 1261 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt