Construct: ORF TRCN0000489633
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021955.1_s317c1
- DNA Barcode:
- CGACCATCTGCTACTTCTGCCCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- KIT (3815)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489633
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3815 | KIT | KIT proto-oncogene, recepto... | NM_000222.2:c.1676T>A | 99.9% | 99.8% | 1961T>C;2290C>T |
| 2 | human | 3815 | KIT | KIT proto-oncogene, recepto... | NM_000222.2:c.1961T>C | 99.9% | 99.8% | 1676T>A;2290C>T |
| 3 | human | 3815 | KIT | KIT proto-oncogene, recepto... | NM_000222.2 | 99.8% | 99.7% | 1676T>A;1961T>C;2290C>T |
| 4 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_005265740.1 | 99.7% | 99.6% | (many diffs) |
| 5 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_017008178.1 | 99.7% | 99.6% | (many diffs) |
| 6 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_005265741.1 | 99.6% | 99.5% | (many diffs) |
| 7 | human | 3815 | KIT | KIT proto-oncogene, recepto... | NM_001093772.1 | 99.4% | 99.3% | (many diffs) |
| 8 | human | 3815 | KIT | KIT proto-oncogene, recepto... | NM_000222.2:c.1673_1687del | 99.4% | 99.3% | 1670_1671insGAAGGATGTTGAGGA;1946T>C;2275C>T |
| 9 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_005265742.3 | 99.3% | 99.2% | (many diffs) |
| 10 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_017008180.1 | 99.3% | 99.2% | (many diffs) |
| 11 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_017008179.1 | 99.2% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2997
- ORF length:
- 2928
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gagaggcgct cgcggcgcct gggattttct ctgcgttctg ctcctactgc 121 ttcgcgtcca gacaggctct tctcaaccat ctgtgagtcc aggggaaccg tctccaccat 181 ccatccatcc aggaaaatca gacttaatag tccgcgtggg cgacgagatt aggctgttat 241 gcactgatcc gggctttgtc aaatggactt ttgagatcct ggatgaaacg aatgagaata 301 agcagaatga atggatcacg gaaaaggcag aagccaccaa caccggcaaa tacacgtgca 361 ccaacaaaca cggcttaagc aattccattt atgtgtttgt tagagatcct gccaagcttt 421 tccttgttga ccgctccttg tatgggaaag aagacaacga cacgctggtc cgctgtcctc 481 tcacagaccc agaagtgacc aattattccc tcaaggggtg ccaggggaag cctcttccca 541 aggacttgag gtttattcct gaccccaagg cgggcatcat gatcaaaagt gtgaaacgcg 601 cctaccatcg gctctgtctg cattgttctg tggaccagga gggcaagtca gtgctgtcgg 661 aaaaattcat cctgaaagtg aggccagcct tcaaagctgt gcctgttgtg tctgtgtcca 721 aagcaagcta tcttcttagg gaaggggaag aattcacagt gacgtgcaca ataaaagatg 781 tgtctagttc tgtgtactca acgtggaaaa gagaaaacag tcagactaaa ctacaggaga 841 aatataatag ctggcatcac ggtgacttca attatgaacg tcaggcaacg ttgactatca 901 gttcagcgag agttaatgat tctggagtgt tcatgtgtta tgccaataat acttttggat 961 cagcaaatgt cacaacaacc ttggaagtag tagataaagg attcattaat atcttcccca 1021 tgataaacac tacagtattt gtaaacgatg gagaaaatgt agatttgatt gttgaatatg 1081 aagcattccc caaacctgaa caccagcagt ggatctatat gaacagaacc ttcactgata 1141 aatgggaaga ttatcccaag tctgagaatg aaagtaatat cagatacgta agtgaacttc 1201 atctaacgag attaaaaggc accgaaggag gcacttacac attcctagtg tccaattctg 1261 acgtcaatgc tgccatagca tttaatgttt atgtgaatac aaaaccagaa atcctgactt 1321 acgacaggct cgtgaatggc atgctccaat gtgtggcagc aggattccca gagcccacaa 1381 tagattggta tttttgtcca ggaactgagc agagatgctc tgcttctgta ctgccagtgg 1441 atgtgcagac actaaactca tctgggccac cgtttggaaa gctagtggtt cagagttcta 1501 tagattctag tgcattcaag cacaatggca cggttgaatg taaggcttac aacgatgtgg 1561 gcaagacttc tgcctatttt aactttgcat ttaaaggtaa caacaaagag caaatccatc 1621 cccacaccct gttcactcct ttgctgattg gtttcgtaat cgtagctggc atgatgtgca 1681 ttattgtgat gattctgacc tacaaatatt tacagaaacc catgtatgaa gtacagtgga 1741 aggatgttga ggagataaat ggaaacaatt atgtttacat agacccaaca caacttcctt 1801 atgatcacaa atgggagttt cccagaaaca ggctgagttt tgggaaaacc ctgggtgctg 1861 gagctttcgg gaaggttgtt gaggcaactg cttatggctt aattaagtca gatgcggcca 1921 tgactgtcgc tgtaaagatg ctcaagccga gtgcccattt gacagaacgg gaagccctca 1981 tgtctgaact caaagtcctg agttaccttg gtaatcacat gaatattgcg aatctacttg 2041 gagcctgcac cattggaggg cccaccctgg tcattacaga atattgttgc tatggtgatc 2101 ttttgaattt tttgagaaga aaacgtgatt catttatttg ttcaaagcag gaagatcatg 2161 cagaagctgc actttataag aatcttctgc attcaaagga gtcttcctgc agcgatagta 2221 ctaatgagta catggacatg aaacctggag tttcttatgt tgtcccaacc aaggccgaca 2281 aaaggagatc tgtgagaata ggctcataca tagaaagaga tgtgactccc gccatcatgg 2341 aggatgacga gttggcctta gacttagaag acttgctgag cttttcttac caggtggcaa 2401 agggcatggc tttcctcgcc tccaagaatt gtattcacag agacttggca gccagaaata 2461 tcctccttac tcatggtcgg atcacaaaga tttgtgattt tggtctagcc agagacatca 2521 agaatgattc taattatgtg gttaaaggaa acgctcgact acctgtgaag tggatggcac 2581 ctgaaagcat tttcaactgt gtatacacgt ttgaaagtga cgtctggtcc TATGGGATTT 2641 TTCTTTGGGA GCTGTTCTCT TTAGGAAGCA GCCCCTATCC TGGAATGCCG GTCGATTCTA 2701 AGTTCTACAA GATGATCAAG GAAGGCTTCC GGATGCTCAG CCCTGAACAC GCACCTGCTG 2761 AAATGTATGA CATAATGAAG ACTTGCTGGG ATGCAGATCC CCTAAAAAGA CCAACATTCA 2821 AGCAAATTGT TCAGCTAATT GAGAAGCAGA TTTCAGAGAG CACCAATCAT ATTTACTCCA 2881 ACTTAGCAAA CTGCAGCCCC AACCGACAGA AGCCCGTGGT AGACCATTCT GTGCGGATCA 2941 ATTCTGTCGG CAGCACCGCT TCCTCCTCCC AGCCTCTGCT TGTGCACGAC GATGTCTAGA 3001 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 3061 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 3121 TTTATATATC TTGTGGAAAG GACGACGACC ATCTGCTACT TCTGCCCTCA CGCGTTAAGT 3181 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt