Construct: ORF TRCN0000489727
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021866.1_s317c1
- DNA Barcode:
- GTTATTCTTCCCTTCGATCTCCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CHRM1 (1128)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489727
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1128 | CHRM1 | cholinergic receptor muscar... | NM_000738.3 | 99.8% | 99.7% | 1198_1199delTGinsGC |
| 2 | human | 1128 | CHRM1 | cholinergic receptor muscar... | XM_011544742.2 | 99.8% | 99.7% | 1198_1199delTGinsGC |
| 3 | mouse | 12669 | Chrm1 | cholinergic receptor, musca... | NM_001112697.1 | 91.3% | 98.6% | (many diffs) |
| 4 | mouse | 12669 | Chrm1 | cholinergic receptor, musca... | NM_007698.3 | 91.3% | 98.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1452
- ORF length:
- 1380
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaacact tcagccccac ctgctgtcag ccccaacatc accgtcctgg 121 caccaggaaa gggtccctgg caagtggcct tcattgggat caccacgggc ctcctgtcgc 181 tagccacagt gacaggcaac ctgctggtac tcatctcttt caaggtcaac acggagctca 241 agacagtcaa taactacttc ctgctgagcc tggcctgtgc tgacctcatc atcggtacct 301 tctccatgaa cctctatacc acgtacctgc tcatgggcca ctgggctctg ggcacgctgg 361 cttgtgacct ctggctggcc ctggactatg tggccagcaa tgcctccgtc atgaatctgc 421 tgctcatcag ctttgaccgc tacttctccg tgactcggcc cctgagctac cgtgccaagc 481 gcacaccccg ccgggcagct ctgatgatcg gcctggcctg gctggtttcc tttgtgctct 541 gggccccagc catcctcttc tggcagtacc tggtagggga gcggacagtg ctagctgggc 601 agtgctacat ccagttcctc tcccagccca tcatcacctt tggcacagcc atggctgcct 661 tctacctccc tgtcacagtc atgtgcacgc tctactggcg catctaccgg gagacagaga 721 accgagcacg ggagctggca gcccttcagg gctccgagac gccaggcaaa gggggtggca 781 gcagcagcag ctcagagagg tctcagccag gggctgaggg ctcaccagag actcctccag 841 gccgctgctg tcgctgctgc cgggccccca ggctgctgca ggcctacagc tggaaggaag 901 aagaggaaga ggacgaaggc tccatggagt ccctcacatc ctcagaggga gaggagcctg 961 gctccgaagt ggtgatcaag atgccaatgg tggaccccga ggcacaggcc cccaccaagc 1021 agcccccacg gagctcccca aatacagtca agaggccgac taagaaaggg cgtgatcgag 1081 ctggcaaggg CCAGAAGCCC CGTGGAAAGG AGCAGCTGGC CAAGCGGAAG ACCTTCTCGC 1141 TGGTCAAGGA GAAGAAGGCG GCTCGGACCC TGAGTGCCAT CCTCCTGGCC TTCATCCTCA 1201 CCTGGACACC GTACAACATC ATGGTGCTGG TGTCCACCTT CTGCAAGGAC TGTGTTCCCG 1261 AGACCCTGGC GGAGCTGGGC TACTGGCTGT GCTACGTCAA CAGCACCATC AACCCCATGT 1321 GCTACGCACT CTGCAACAAA GCCTTCCGGG ACACCTTTCG CCTGCTGCTG CTTTGCCGCT 1381 GGGACAAGAG ACGCTGGCGC AAGATCCCCA AGCGCCCTGG CTCCGTGCAC CGCACTCCCT 1441 CCCGCCAATG CTGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1501 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1561 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTTATTCTTC CCTTCGATCT 1621 CCCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt