Construct: ORF TRCN0000489755
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020888.1_s317c1
- DNA Barcode:
- TGCCGGGGTCAAGGCAAAAATGAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LGR4 (55366)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489755
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55366 | LGR4 | leucine rich repeat contain... | NM_018490.5 | 99.9% | 100% | 2181A>G |
| 2 | human | 55366 | LGR4 | leucine rich repeat contain... | NM_001346432.1 | 97.4% | 97.4% | 182_183ins72 |
| 3 | mouse | 107515 | Lgr4 | leucine-rich repeat-contain... | NM_172671.2 | 89.3% | 92.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2925
- ORF length:
- 2853
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgccgggc ccgctagggc tgctctgctt cctcgccctg gggctgctcg 121 gctcggccgg gcccagcggc gcggcgccgc ctctctgcgc ggcgccctgc agctgcgacg 181 gcgaccgtcg ggtggactgc tccgggaagg ggctgacggc cgtgcccgag gggctcagcg 241 ccttcaccca agcgctggat atcagtatga acaacattac tcagttgcca gaagatgcat 301 ttaagaactt tccttttcta gaagagctac aattggcggg caacgacctt tcttttatcc 361 acccaaaggc cttgtctggg ttgaaagaac tcaaagttct aacgctccag aataatcagt 421 tgaaaacagt acccagtgaa gccattcgag ggctgagtgc tttgcagtct ttgcgtttag 481 atgccaacca tattacctca gtccccgagg acagttttga aggacttgtt cagttacggc 541 atctgtggct ggatgacaac agcttgacgg aggtgcctgt gcaccccctc agcaatctgc 601 ccaccctaca ggcgctgacc ctggctctca acaagatctc aagcatccct gactttgcat 661 ttaccaacct ttcaagcctg gtagttctgc atcttcataa caataaaatt agaagcctga 721 gtcaacactg ttttgatgga ctagataacc tggagacctt agacttgaat tataataact 781 tgggggaatt tcctcaggct attaaagccc ttcctagcct taaagagcta ggatttcata 841 gtaattctat ttctgttatc cctgatggag catttgatgg taatccactc ttaagaacta 901 tacatttgta tgataatcct ctgtcttttg tggggaactc agcatttcac aatttatctg 961 atcttcattc cctagtcatt cgtggtgcaa gcatggtgca gcagttcccc aatcttacag 1021 gaactgtcca cctggaaagt ctgactttga caggtacaaa gataagcagc atacctaata 1081 atttgtgtca agaacaaaag atgcttagga ctttggactt gtcttacaat aatataagag 1141 accttccaag ttttaatggt tgccatgctc tggaagaaat ttctttacag cgtaatcaaa 1201 tctaccaaat aaaggaaggc acctttcaag gcctgatatc tctaaggatt ctagatctga 1261 gtagaaacct gatacatgaa attcacagta gagcttttgc cacacttggg ccaataacta 1321 acctagatgt aagtttcaat gaattaactt cctttcctac ggaaggcctg aatgggctaa 1381 atcaactgaa acttgtgggc aacttcaagc tgaaagaagc cttagcagca aaagactttg 1441 ttaacctcag gtctttatca gtaccatatg cttatcagtg ctgtgcattt tggggttgtg 1501 actcttatgc aaatttaaac acagaagata acagcctcca ggaccacagt gtggcacagg 1561 agaaaggtac tgctgatgca gcaaatgtca caagcactct tgaaaatgaa gaacatagtc 1621 aaataattat ccattgtaca ccttcaacag gtgcttttaa gccctgtgaa tatttactgg 1681 gaagctggat gattcgtctt actgtgtggt tcattttctt ggttgcatta tttttcaacc 1741 tgcttgttat tttaacaaca tttgcatctt gtacatcact gccttcgtcc aaattgttta 1801 taggcttgat ttctgtgtct aacttattca tgggaatcta tactggcatc ctaacttttc 1861 ttgatgctgt gtcctggggc agattcgctg aatttggcat ttggtgggaa actggcagtg 1921 gctgcaaagt agctgggttt cttgcagttt tctcctcaga aagtgccata tttttattaa 1981 tgctagcaac tgtcgaaaga agcttatctg caaaagatat aatgaaaaat gggaagagca 2041 atcatctcaa acagttccgg gttgctgccc ttttggcttt cctaggtgct acagtagcag 2101 gctgttttcc ccttttccat agaggggaat attctgcatc acccctttgt ttgccatttc 2161 ctacaggtga aacgccatca ttaggattca ctgtaacgtt agtgctatta aactcactag 2221 catttttatt aatggccgtt atctacacta agctatactg caacttggaa aaagaggacc 2281 tctcagaaaa ctcacaatct agcatgatta agcatgtcgc ttggctaatc ttcaccaatt 2341 gcatcttttt ctgccctgtg gcgttttttt catttgcacc attgatcact gcaatctcta 2401 tcagccccga aataatgaag tctgttactc tgatattttt tccattgcct gcttgcctga 2461 atccagtcct gtatgttttc ttcaacccaa agtttaaaga aGACTGGAAG TTACTGAAGC 2521 GACGTGTTAC CAAGAAAAGT GGATCAGTTT CAGTTTCCAT CAGTAGCCAA GGTGGTTGTC 2581 TGGAACAGGA TTTCTACTAC GACTGTGGCA TGTACTCACA TTTGCAGGGC AACCTGACTG 2641 TTTGCGACTG CTGCGAATCG TTTCTTTTAA CAAAGCCAGT ATCATGCAAA CACTTGATAA 2701 AATCACACAG CTGTCCTGCA TTGGCAGTGG CTTCTTGCCA AAGACCTGAG GGCTACTGGT 2761 CCGACTGTGG CACACAGTCG GCCCACTCTG ATTATGCAGA TGAAGAAGAT TCCTTTGTCT 2821 CAGACAGTTC TGACCAGGTG CAGGCCTGTG GACGAGCCTG CTTCTACCAG AGTAGAGGAT 2881 TCCCTTTGGT GCGCTATGCT TACAATCTAC CAAGAGTTAA AGACTAGGAC CCAGCTTTCT 2941 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 3001 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 3061 GTGGAAAGGA CGATGCCGGG GTCAAGGCAA AAATGACACG CGTTAAGTCg acaatcaacc 3121 tctggattac aaaatttgtg aaagatt