Construct: ORF TRCN0000489761
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020209.1_s317c1
- DNA Barcode:
- GACATAGGAATATCACTTTGTATA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PANK3 (79646)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489761
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79646 | PANK3 | pantothenate kinase 3 | NM_024594.4 | 100% | 100% | |
| 2 | mouse | 211347 | Pank3 | pantothenate kinase 3 | NM_145962.2 | 93.3% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1182
- ORF length:
- 1110
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaagatc aaagatgcca agaaaccctc tttcccatgg tttggcatgg 121 acattggggg aactctagta aagctctcgt actttgaacc tattgatatc acagcagagg 181 aagagcaaga agaagttgag agtttaaaaa gtattcggaa atatttgact tctaacgtgg 241 catatggatc caccggcatt cgggatgtac accttgaact gaaagattta acactttttg 301 gccgaagagg gaacttgcac tttatcaggt ttccaaccca ggacctgcct acttttatcc 361 aaatgggaag agataaaaac ttctcaacat tgcagacggt gctatgtgct acaggaggtg 421 gtgcttacaa gtttgaaaaa gattttcgca caattggaaa cctccacctg cacaaactgg 481 atgaacttga ctgccttgta aagggcttgc tgtatataga ctctgtcagt ttcaatggac 541 aagccgagtg ctattatttt gctaatgcct cagaacctga gcgatgccaa aagatgcctt 601 ttaacctgga tgatccctat ccactgcttg tagtgaacat tggctcagga gtcagtattt 661 tagcagtcca ttccaaagac aactataaac gagtgactgg gacaagcctt ggagggggta 721 cctttctggg tttatgcagt ttattgactg gctgtgaaag ttttgaagag gctcttgaaa 781 tggcatccaa aggtgatagc acacaagctg acaagctggT CCGTGATATT TATGGAGGAG 841 ATTATGAAAG ATTTGGTTTG CCAGGTTGGG CTGTAGCATC TAGTTTTGGG AATATGATTT 901 ATAAGGAGAA GCGAGAATCT GTTAGTAAAG AAGATCTGGC AAGAGCTACT TTAGTTACTA 961 TCACCAATAA CATTGGTTCT GTGGCACGAA TGTGTGCTGT TAATGAGAAA ATAAACAGAG 1021 TTGTCTTTGT TGGAAACTTT TTACGTGTCA ATACCCTCTC AATGAAACTT TTGGCATATG 1081 CACTGGATTA CTGGTCAAAA GGTCAACTAA AAGCATTGTT TCTAGAACAT GAGGGTTACT 1141 TTGGAGCAGT TGGTGCACTT CTTGGGCTGC CAAATTTCAG CTGAGACCCA GCTTTCTTGT 1201 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1261 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1321 GAAAGGACGA GACATAGGAA TATCACTTTG TATAACGCGT TAAGTCgaca atcaacctct 1381 ggattacaaa atttgtgaaa gatt