Construct: ORF TRCN0000489790
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019575.1_s317c1
- DNA Barcode:
- TGTACAGATAGGTATAAAGCTCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADGRL4 (64123)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489790
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64123 | ADGRL4 | adhesion G protein-coupled ... | NM_022159.4 | 87.7% | 87.5% | 1_252del;898G>T;2070_2071insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1896
- ORF length:
- 1821
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgtgt gtacctggct tcagatccag cagtaaccaa gacaggttta 121 tcactaatga tggaaccgtc tgtatagaaa atgtgaatgc aaactgccat ttagataatg 181 tctgtatagc tgcaaatatt aataaaactt taacaaaaat cagatccata aaagaacctg 241 tggctttgct acaagaagtc tatagaaatt ctgtgacaga tctttcacca acagatataa 301 ttacatatat agaaatatta gctgaatcat cttcattact aggttacaag aacaacacta 361 tctcagccaa ggacaccctt tctaactcaa ctcttactga atttgtaaaa accgtgaata 421 attttgttca aagggataca tttgtagttt gggacaagtt atctgtgaat cataggagaa 481 cacatcttac aaaactcatg cacactgttg aacaagctac tttaaggata tcccagagct 541 tccaaaagac cacagagttt gatacaaatt caacggatat agctctcaaa gttttctttt 601 ttgattcata taacatgaaa catattcatc ctcatatgaa tatggatgga gactacataa 661 atatatttcc aaagagaaaa gctgcatatg attcaaatgg caatgttgca gttgcatttt 721 tatattataa gagtattggt cctttgcttt catcatctga caacttctta ttgaaacctc 781 aaaattatga taattctgaa gaggaggaaa gagtcatatc ttcagtaatt tcagtctcaa 841 tgagctcaaa cccacccaca ttatatgaac ttgaaaaaat aacatttaca ttaagtcatc 901 gaaaggtcac agataggtat aggagtctat gtgcattttg gaattactca cctgatacca 961 tgaatggcag ctggtcttca gagggctgtg agctgacata ctcaaatgag acccacacct 1021 catgccgctg taatcacctg acacattttg caattttgat gtcctctggt ccttccattg 1081 gtattaaaga ttataatatt cttacaagga tcactcaact aggaataatt atttcactga 1141 tttgtcttgc catatgcatt tttaccttct ggttcttcag tgaaattcaa agcaccagga 1201 caacaattca caaaaatctt tgctgtagcc tatttcttgc tgaacttgtt tttcttgttg 1261 ggatcaatac aaatactaat aagctcttct gttcaatcat tgccggactg ctacactact 1321 tctttttagc tgcttttgca tggatgtgca ttgaaggcat acatctctat ctcattgttg 1381 tgggtgtcat ctacaacaag ggatttttgc acaagaattt ttatatcttt ggctatctaa 1441 gcccagccgt ggtagttgga ttttcggcag cactaggata cagatattat ggcacaacca 1501 aagtatgttg gcttagcacc gaaaacaact ttatttggag ttttatagga ccagcatgcc 1561 taatcattct tgttaatctc ttggcttttG GAGTCATCAT ATACAAAGTT TTTCGTCACA 1621 CTGCAGGGTT GAAACCAGAA GTTAGTTGCT TTGAGAACAT AAGGTCTTGT GCAAGAGGAG 1681 CCCTCGCTCT TCTGTTCCTT CTCGGCACCA CCTGGATCTT TGGGGTTCTC CATGTTGTGC 1741 ACGCATCAGT GGTTACAGCT TACCTCTTCA CAGTCAGCAA TGCTTTCCAG GGGATGTTCA 1801 TTTTTTTATT CCTGTGTGTT TTATCTAGAA AGATTCAAGA AGAATATTAC AGATTGTTCA 1861 AAAATGTCCC CTGTTGTTTT GGATGTTTAA GGGACCCAGC TTTCTTGTAC AAAGTGGTTG 1921 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1981 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 2041 TACAGATAGG TATAAAGCTC GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 2101 ttgtgaaaga tt