Construct: ORF TRCN0000489872
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020684.1_s317c1
- DNA Barcode:
- TGCACACCTTCCTAATAAGTGCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TWF2 (11344)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489872
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11344 | TWF2 | twinfilin actin binding pro... | NM_007284.4 | 100% | 100% | |
| 2 | mouse | 23999 | Twf2 | twinfilin actin binding pro... | NM_011876.3 | 87.9% | 96.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1119
- ORF length:
- 1047
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgcac caaacgggca tccacgccac ggaagagctg aaggaattct 121 ttgccaaggc acgggctggc tctgtgcggc tcatcaaggt tgtgattgag gacgagcagc 181 tcgtgctggg tgcctcgcag gagccagtag gccgctggga tcaggactat gacagggccg 241 tgctgccact gctggacgcc cagcagccct gctacctgct ctaccgcctc gactcacaga 301 atgctcaggg cttcgaatgg ctcttcctcg cctggtcgcc tgataactcc cccgtgcggc 361 tgaagatgct gtacgcggcc acgcgggcca cagtgaaaaa ggagtttgga ggtggccaca 421 tcaaggatga gctcttcggg actgtgaagg atgacctctc ttttgctggg taccagaaac 481 acctgtcgtc ctgtgcggca cctgccccgc tgacctcggc tgagagagag ctccagcaga 541 tccgcattaa cgaggtgaag acagagatca gtgtggaaag caagcaccag accctgcagg 601 gcctcgcctt ccccctgcag cctgaggccc agcgggcact ccagcagctc aagcagaaaa 661 tggtcaacta catccagatg aagctggacc tagagcggga aaccattgag ctggtgcaca 721 cagagcccac ggatgtggcc cagctgccct cccgggtgcc ccgagatgct gcccgctacc 781 acttcttcct cTACAAGCAC ACCCATGAGG GCGACCCCCT TGAGTCTGTA GTGTTCATCT 841 ACTCCATGCC GGGGTACAAG TGCAGCATCA AGGAGCGAAT GCTCTACTCC AGCTGCAAGA 901 GCCGCCTCCT CGACTCCGTG GAGCAGGACT TCCATCTGGA GATCGCCAAG AAAATTGAGA 961 TTGGCGATGG GGCAGAGCTG ACGGCAGAGT TCCTCTACGA CGAGGTGCAC CCCAAGCAAC 1021 ACGCCTTCAA GCAGGCCTTC GCCAAGCCCA AGGGCCCAGG GGGCAAGCGG GGCCATAAGC 1081 GCCTCATCCG CGGCCCGGGT GAAAATGGGG ATGACAGCTA GAACCCAGCT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGATGC ACACCTTCCT AATAAGTGCA CACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t