Construct: ORF TRCN0000489933
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021798.1_s317c1
- DNA Barcode:
- AAGCTCAACACACATATGTCCCAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC47A1 (55244)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489933
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55244 | SLC47A1 | solute carrier family 47 me... | NM_018242.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1779
- ORF length:
- 1710
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggaagctcct gaggagcccg cgccagtgcg cggaggcccg gaggccaccc 121 ttgaggtccg tgggtcgcgc tgcttgcggc tgtccgcctt ccgagaagag ctgcgggcgc 181 tcttggtcct ggctggcccc gcgttcttgg ttcagctgat ggtgttcctg atcagcttca 241 taagctccgt gttctgtggc cacctgggca agctggagct ggatgcagtc acgctggcaa 301 tcgcggttat caatgtcact ggtgtctcag tgggattcgg cttatcttct gcctgtgaca 361 ccctcatctc ccagacgtac gggagccaga acctgaagca cgtgggcgtg atcctgcagc 421 ggagtgcgct cgtcctgctc ctctgctgct tcccctgctg ggcgctcttt ctcaacaccc 481 agcacatcct gctgctcttc aggcaggacc cagatgtgtc caggcttacc cagacctatg 541 tcacgatctt cattccagct cttcctgcaa cctttcttta tatgttacaa gttaaatatt 601 tgctcaacca gggaattgta ctgccccaga tcgtaactgg agttgcagcc aaccttgtca 661 atgccctcgc caactatctg tttctccatc aactgcatct tggggtgata ggctctgcac 721 tggcaaactt gatttcccag tacaccctgg ctctactcct ctttctctac atcctcggga 781 aaaaactgca tcaagctaca tggggaggct ggtccctcga gtgcctgcag gactgggcct 841 ccttcctccg cctggccatc cccagcatgc tcatgctgtg catggagtgg tgggcctatg 901 aggtcgggag cttcctcagt ggcatcctcg gcatggtgga gctgggcgct cagtccatcg 961 tgtatgaact ggccatcatt gtgtacatgg tccctgcagg cttcagtgtg gctgccagtg 1021 tccgggtagg aaacgctctg ggtgctggag acatggagca ggcacggaag tcctctaccg 1081 tttccctgct gattacagtg ctctttgctg tagccttcag tgtcctgctg ttaagctgta 1141 aggatcacgt ggggtacatt tttactaccg accgagacat cattaatctg gtggctcagg 1201 tggttccaat ttatgctgtt tcccacctct ttgaagctct tgcttgcacg agtggtggtg 1261 ttctgagggg gagtggaaat cagaaggttg gagccattgt gaataccatt gggtactatg 1321 tggttggcct ccccatcggg atcgcgctga tgtttgcaac cacacttgga gTGATGGGTC 1381 TGTGGTCAGG GATCATCATC TGTACAGTCT TTCAAGCTGT GTGTTTTCTA GGCTTTATTA 1441 TTCAGCTAAA TTGGAAAAAA GCCTGTCAGC AGGCTCAGGT ACACGCCAAT TTGAAAGTAA 1501 ACAACGTGCC TCGGAGTGGG AATTCTGCTC TCCCTCAGGA TCCGCTTCAC CCAGGGTGCC 1561 CTGAAAACCT TGAAGGAATT TTAACGAACG ATGTTGGAAA GACAGGCGAG CCTCAGTCAG 1621 ATCAGCAGAT GCGCCAAGAA GAACCTTTGC CGGAACATCC ACAGGACGGC GCTAAATTGT 1681 CCAGGAAACA GCTGGTGCTG CGGCGAGGGC TTCTGCTCCT GGGGGTCTTC TTAATCTTGC 1741 TGGTGGGGAT TTTAGTGAGA TTCTATGTCA GAATTCAGTA GAACCCAGCT TTCTTGTACA 1801 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1861 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1921 AGGACGAAAG CTCAACACAC ATATGTCCCA GACGCGTTAA GTCgacaatc aacctctgga 1981 ttacaaaatt tgtgaaagat t