Construct: ORF TRCN0000489948
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020381.1_s317c1
- DNA Barcode:
- GAAACGTGAAACTTCAACAACCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NDUFB6 (4712)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489948
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4712 | NDUFB6 | NADH:ubiquinone oxidoreduct... | NM_002493.5 | 100% | 100% | |
| 2 | human | 4712 | NDUFB6 | NADH:ubiquinone oxidoreduct... | NM_182739.3 | 88.2% | 88.2% | 271_272ins45 |
| 3 | human | 4712 | NDUFB6 | NADH:ubiquinone oxidoreduct... | NM_001199987.1 | 75.7% | 75.7% | 180_181ins93 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 89
- ORF end:
- 473
- ORF length:
- 384
- Sequence:
-
1 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG 61 TTTGTACAAA AAAGCAGGCT TCGCCACCAT GACGGGGTAC ACTCCGGATG AGAAACTGCG 121 GCTGCAGCAG CTGCGAGAGC TGAGAAGGCG ATGGCTGAAG GACCAGGAGC TGAGCCCTCG 181 GGAGCCGGTG CTGCCCCCAC AGAAGATGGG GCCTATGGAG AAATTCTGGA ATAAATTTTT 241 GGAGAATAAA TCCCCTTGGA GGAAAATGGT CCATGGGGTA TACAAAAAGA GTATCTTTGT 301 TTTCACTCAT GTACTTGTAC CTGTCTGGAT TATTCATTAT TACATGAAGT ATCATGTTTC 361 TGAAAAACCA TATGGCATAG TTGAAAAGAA GTCCAGAATA TTCCCTGGTG ATACAATTCT 421 GGAGACTGGA GAAGTAATTC CACCAATGAA AGAATTTCCT GATCAACATC ATTAGGACCC 481 AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT 541 CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA 601 TATATCTTGT GGAAAGGACG AGAAACGTGA AACTTCAACA ACCCGACGCG TTAAGTCgac 661 aatcaacctc tggattacaa aatttgtgaa agatt