Construct: ORF TRCN0000491253
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020178.2_s317c1
- DNA Barcode:
- GCAGATTCCGCATCTGCAATCATT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HSPB8 (26353)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491253
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26353 | HSPB8 | heat shock protein family B... | NM_014365.2 | 100% | 100% | |
| 2 | mouse | 80888 | Hspb8 | heat shock protein 8 | NM_030704.3 | 87.4% | 94.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 660
- ORF length:
- 588
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctgac ggtcagatgc ccttctcctg ccactaccca agccgcctgc 121 gccgagaccc cttccgggac tctcccctct cctctcgcct gctggatgat ggctttggca 181 tggacccctt cccagacgac ttgacagcct cttggcccga ctgggctctg cctcgtctct 241 cctccgcctg gccaggcacc ctaaggtcgg gcatggtgcc ccggggcccc actgccaccg 301 ccaggtttgg ggtgcctgcc gagggcagga cccccccacc cttccctggg gagccctgga 361 aagtgtgtgt gaatgtgcac agcttcaagc cagaggagtt gatggtgaag accaaagatG 421 GATACGTGGA GGTGTCTGGC AAACATGAAG AGAAACAGCA AGAAGGTGGC ATTGTTTCTA 481 AGAACTTCAC AAAGAAAATC CAGCTTCCTG CAGAGGTGGA TCCTGTGACA GTATTTGCCT 541 CACTTTCCCC AGAGGGTCTG CTGATCATCG AAGCTCCCCA GGTCCCTCCT TACTCAACAT 601 TTGGAGAGAG CAGTTTCAAC AACGAGCTTC CCCAGGACAG CCAGGAAGTC ACCTGTACCT 661 GAGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 721 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 781 GGCTTTATAT ATCTTGTGGA AAGGACGAGC AGATTCCGCA TCTGCAATCA TTACGCGTTA 841 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt