Construct: ORF TRCN0000491272
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015450.1_s317c1
- Derived from:
- ccsbBroadEn_00180
- DNA Barcode:
- TTCACAGGCGGTCTGCGCCCGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BTK (695)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491272
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 695 | BTK | Bruton tyrosine kinase | NM_000061.2 | 100% | 100% | |
| 2 | human | 695 | BTK | Bruton tyrosine kinase | NM_001287344.1 | 95% | 95% | 1_102del |
| 3 | human | 695 | BTK | Bruton tyrosine kinase | NM_001287345.1 | 73.2% | 73.2% | 1033_1034ins528 |
| 4 | mouse | 12229 | Btk | Bruton agammaglobulinemia t... | NM_013482.2 | 91.5% | 98.3% | (many diffs) |
| 5 | mouse | 12229 | Btk | Bruton agammaglobulinemia t... | XM_006528483.3 | 91.5% | 98.3% | (many diffs) |
| 6 | mouse | 12229 | Btk | Bruton agammaglobulinemia t... | XM_006528484.3 | 91.5% | 98.3% | (many diffs) |
| 7 | mouse | 12229 | Btk | Bruton agammaglobulinemia t... | XM_006528485.3 | 91.5% | 98.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2046
- ORF length:
- 1977
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccgcagtg attctggaga gcatctttct gaagcgatcc caacagaaaa 121 agaaaacatc acctctaaac ttcaagaagc gcctgtttct cttgaccgtg cacaaactct 181 cctactatga gtatgacttt gaacgtggga gaagaggcag taagaagggt tcaatagatg 241 ttgagaagat cacttgtgtt gaaacagtgg ttcctgaaaa aaatcctcct ccagaaagac 301 agattccgag aagaggtgaa gagtccagtg aaatggagca aatttcaatc attgaaaggt 361 tcccttatcc cttccaggtt gtatatgatg aagggcctct ctacgtcttc tccccaactg 421 aagaactaag gaagcggtgg attcaccagc tcaaaaacgt aatccggtac aacagtgatc 481 tggttcagaa atatcaccct tgcttctgga tcgatgggca gtatctctgc tgctctcaga 541 cagccaaaaa tgctatgggc tgccaaattt tggagaacag gaatggaagc ttaaaacctg 601 ggagttctca ccggaagaca aaaaagcctc ttcccccaac gcctgaggag gaccagatct 661 tgaaaaagcc actaccgcct gagccagcag cagcaccagt ctccacaagt gagctgaaaa 721 aggttgtggc cctttatgat tacatgccaa tgaatgcaaa tgatctacag ctgcggaagg 781 gtgatgaata ttttatcttg gaggaaagca acttaccatg gtggagagca cgagataaaa 841 atgggcagga aggctacatt cctagtaact atgtcactga agcagaagac tccatagaaa 901 tgtatgagtg gtattccaaa cacatgactc ggagtcaggc tgagcaactg ctaaagcaag 961 aggggaaaga aggaggtttc attgtcagag actccagcaa agctggcaaa tatacagtgt 1021 ctgtgtttgc taaatccaca ggggaccctc aaggggtgat acgtcattat gttgtgtgtt 1081 ccacacctca gagccagtat tacctggctg agaagcacct tttcagcacc atccctgagc 1141 tcattaacta ccatcagcac aactctgcag gactcatatc caggctcaaa tatccagtgt 1201 ctcaacaaaa caagaatgca ccttccactg caggcctggg atacggatca tgggaaattg 1261 atccaaagga cctgaccttc ttgaaggagc tggggactgg acaatttggg gtagtgaagt 1321 atgggaaatg gagaggccag tacgacgtgg ccatcaagat gatcaaagaa ggctccatgt 1381 ctgaagatga attcattgaa gaagccaaag tcatgatgaa tctttcccat gagaagctgg 1441 tgcagttgta tggcgtctgc accaagcagc gccccatctt catcatcact gagtacatgg 1501 ccaatggctg cctcctgaac tacctgaggg agatgcgcca ccgcttccag actcagcagc 1561 tgctagagat gtgcaaggat gtctgtgaag ccatggaata cctggagtca aagcagttcc 1621 ttcaccgaga cctggcagct cgaaactgtt tggtaaacga tcaaggagtt gttaaagtat 1681 ctgatttcgg cctgtccagg tatgtcctgg atgatgaata cacaagctca gtaggctcca 1741 aatttccagt ccggtggtcc ccaccggaag tccTGATGTA TAGCAAGTTC AGCAGCAAAT 1801 CTGACATTTG GGCTTTTGGG GTTTTGATGT GGGAAATTTA CTCCCTGGGG AAGATGCCAT 1861 ATGAGAGATT TACTAACAGT GAGACTGCTG AACACATTGC CCAAGGCCTA CGTCTCTACA 1921 GGCCTCATCT GGCTTCAGAG AAGGTATATA CCATCATGTA CAGTTGCTGG CATGAGAAAG 1981 CAGATGAGCG TCCCACTTTC AAAATTCTTC TGAGCAATAT TCTAGATGTC ATGGATGAAG 2041 AATCCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2101 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2161 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTCACAGGC GGTCTGCGCC CGGTGACGCG 2221 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt