Construct: ORF TRCN0000491301
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013150.2_s317c1
- Derived from:
- ccsbBroadEn_04692
- DNA Barcode:
- TTTGCGGAAGTTTCTGTCACCAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DIRC1 (116093)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491301
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116093 | DIRC1 | disrupted in renal carcinoma 1 | NR_161166.1 | 21.1% | 1_290del;603_1473del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 381
- ORF length:
- 312
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctgaggcc cacatgcagc ctgcaaaact gcaaacatca ctgcccacca 121 cagaccacgg aagcaagaag cctgtttcct gttacttgcc accactcagc aatgcccacc 181 ccatgtGTAT AGAGGTCCAG AATGCTCAAA ATTGTTCTTC TGCTGCTGCA ACATTGGAGC 241 CTTCCATCAT CTCAGACACA TGCTTTTATA AACCAATAAC AAAGGATCAA CTTTCTTCTA 301 GGTCTGAATT GAATACTGTG AGGCTAAAGT GTCTTAACTC ATTGAGGGGG TGGAAAATAT 361 TAAACCAACT AAGCCTTACC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 421 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 481 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTTG CGGAAGTTTC 541 TGTCACCAGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt