Construct: ORF TRCN0000491330
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021397.2_s317c1
- DNA Barcode:
- GCCGAGCGGATCGATCCCAGCGGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CLK3 (1198)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491330
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1198 | CLK3 | CDC like kinase 3 | NM_003992.4 | 99.4% | 100% | 1470_1471insTGAAAGCT |
| 2 | human | 1198 | CLK3 | CDC like kinase 3 | NM_001130028.1 | 76.4% | 76.8% | 1_444del;1914_1915insTGAAAGCT |
| 3 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_006510739.3 | 92.2% | 98.7% | (many diffs) |
| 4 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313060.1 | 92.2% | 98.7% | (many diffs) |
| 5 | mouse | 102414 | Clk3 | CDC-like kinase 3 | NM_007713.4 | 70.9% | 75.8% | (many diffs) |
| 6 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313061.1 | 51.4% | 54.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1536
- ORF length:
- 1470
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat 61 tgaccatgca tcactgtaag cgataccgct cccctgaacc agacccgtac ctgagctacc 121 gatggaagag gaggaggtcc tacagtcggg aacatgaagg gagactgcga tacccgtccc 181 gaagggagcc tcccccacga agatctcggt ccagaagcca tgaccgcctg ccctaccaga 241 ggaggtaccg ggagcgccgt gacagcgata cataccggtg tgaagagcgg agcccatcct 301 ttggagagga ctactatgga ccttcacgtt ctcgtcatcg tcggcgatcg cgggagaggg 361 ggccataccg gacccgcaag catgcccacc actgccacaa acgccgcacc aggtcttgta 421 gcagcgcctc ctcgagaagc caacagagca gtaagcgcag cagccggagt gtggaagatg 481 acaaggaggg tcacctggtg tgccggatcg gcgattggct ccaagagcga tatgagattg 541 tggggaacct gggtgaaggc acctttggca aggtggtgga gtgcttggac catgccagag 601 ggaagtctca ggttgccctg aagatcatcc gcaacgtggg caagtaccgg gaggctgccc 661 ggctagaaat caacgtgctc aaaaaaatca aggagaagga caaagaaaac aagttcctgt 721 gtgtcttgat gtctgactgg ttcaacttcc acggtcacat gtgcatcgcc tttgagctcc 781 tgggcaagaa cacctttgag ttcctgaagg agaataactt ccagccttac cccctaccac 841 atgtccggca catggcctac cagctctgcc acgcccttag atttctgcat gagaatcagc 901 tgacccatac agacttgaaa ccagagaaca tcctgtttgt gaattctgag tttgaaaccc 961 tctacaatga gcacaagagc tgtgaggaga agtcagtgaa gaacaccagc atccgagtgg 1021 ctgactttgg cagtgccaca tttgaccatg agcaccacac caccattgtg gccacccgtc 1081 actatcgccc gcctgaggtg atccttgagc tgggctgggc acagccctgt gacgtctgga 1141 gcattggctg cattctcttt gagtactacc ggggcttcac actcttccag acccacgaaa 1201 accgagagca cctggtgatg atggagaaga tcctagggcc catcccatca cacatgatcc 1261 accgtaccag gaagcagaaa tatttctaca aagggggcct agtttGGGAT GAGAACAGCT 1321 CTGACGGCCG GTATGTGAAG GAGAACTGCA AACCTCTGAA GAGTTACATG CTCCAAGACT 1381 CCCTGGAGCA CGTGCAGCTG TTTGACCTGA TGAGGAGGAT GTTAGAATTT GACCCTGCCC 1441 AGCGCATCAC ACTGGCCGAG GCCCTGCTGC ACCCCTTCTT TGCTGGCCTG ACCCCTGAGG 1501 AGCGGTCCTT CCACACCAGC CGCAACCCAA GCAGATGAAA GCTTGACCCA GCTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA GCCGAGCGGA TCGATCCCAG CGGTACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt