Construct: ORF TRCN0000491339
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020515.2_s317c1
- DNA Barcode:
- ATTCTCCCAACGTCGAAGGAAGAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CLK3 (1198)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491339
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1198 | CLK3 | CDC like kinase 3 | NM_003992.4 | 95.3% | 95.3% | 470_538del |
| 2 | human | 1198 | CLK3 | CDC like kinase 3 | NM_001130028.1 | 73.1% | 73.1% | 1_444del;914_982del |
| 3 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_006510739.3 | 88% | 94% | (many diffs) |
| 4 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313060.1 | 88% | 94% | (many diffs) |
| 5 | mouse | 102414 | Clk3 | CDC-like kinase 3 | NM_007713.4 | 67.6% | 72.2% | (many diffs) |
| 6 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313061.1 | 54.2% | 57.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1473
- ORF length:
- 1401
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcatcac tgtaagcgat accgctcccc tgaaccagac ccgtacctga 121 gctaccgatg gaagaggagg aggtcctaca gtcgggaaca tgaagggaga ctgcgatacc 181 cgtcccgaag ggagcctccc ccacgaagat ctcggtccag aagccatgac cgcctgccct 241 accagaggag gtaccgggag cgccgtgaca gcgatacata ccggtgtgaa gagcggagcc 301 catcctttgg agaggactac tatggacctt cacgttctcg tcatcgtcgg cgatcgcggg 361 agagggggcc ataccggacc cgcaagcatg cccaccactg ccacaaacgc cgcaccaggt 421 cttgtagcag cgcctcctcg agaagccaac agagcagtaa gcgcagcagc cggagtgtgg 481 aagatgacaa ggagggtcac ctggtgtgcc ggatcggcga ttggctccaa gagcgatatg 541 agtctcaggt tgccctgaag atcatccgca acgtgggcaa gtaccgggag gctgcccggc 601 tagaaatcaa cgtgctcaaa aaaatcaagg agaaggacaa agaaaacaag ttcctgtgtg 661 tcttgatgtc tgactggttc aacttccacg gtcacatgtg catcgccttt gagctcctgg 721 gcaagaacac ctttgagttc ctgaaggaga ataacttcca gccttacccc ctaccacatg 781 tccggcacat ggcctaccag ctctgccacg cccttagatt tctgcatgag aatcagctga 841 cccatacaga cttgaaacca gagaacatcc tgtttgtgaa ttctgagttt gaaaccctct 901 acaatgagca caagagctgt gaggagaagt cagtgaagaa caccagcatc cgagtggctg 961 actttggcag tgccacattt gaccatgagc accacaccac cattgtggcc acccgtcact 1021 atcgcccgcc tgaggtgatc cttgagctgg gctgggcaca gccctgtgac gtctggagca 1081 ttGGCTGCAT TCTCTTTGAG TACTACCGGG GCTTCACACT CTTCCAGACC CACGAAAACC 1141 GAGAGCACCT GGTGATGATG GAGAAGATCC TAGGGCCCAT CCCATCACAC ATGATCCACC 1201 GTACCAGGAA GCAGAAATAT TTCTACAAAG GGGGCCTAGT TTGGGATGAG AACAGCTCTG 1261 ACGGCCGGTA TGTGAAGGAG AACTGCAAAC CTCTGAAGAG TTACATGCTC CAAGACTCCC 1321 TGGAGCACGT GCAGCTGTTT GACCTGATGA GGAGGATGTT AGAATTTGAC CCTGCCCAGC 1381 GCATCACACT GGCCGAGGCC CTGCTGCACC CCTTCTTTGC TGGCCTGACC CCTGAGGAGC 1441 GGTCCTTCCA CACCAGCCGC AACCCAAGCA GATAGAACCC AGCTTTCTTG TACAAAGTGG 1501 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1561 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1621 AATTCTCCCA ACGTCGAAGG AAGAGACGCG TTAAGTCgac aatcaacctc tggattacaa 1681 aatttgtgaa agatt