Construct: ORF TRCN0000491405
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018188.2_s317c1
- Derived from:
- ccsbBroadEn_01985
- DNA Barcode:
- TATAGGTCTAACGCGAATTGTGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF3H (8667)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491405
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8667 | EIF3H | eukaryotic translation init... | NM_003756.3 | 100% | 100% | |
| 2 | mouse | 68135 | Eif3h | eukaryotic translation init... | NM_080635.1 | 89.5% | 97.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1122
- ORF length:
- 1056
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcccgcaag gaaggtaccg gctctactgc cacctcttcc agctccaccg 121 ccggcgcagc agggaaaggc aaaggcaaag gcggctcggg agattcagcc gtgaagcaag 181 tgcagataga tggccttgtg gtattaaaga taatcaaaca ttatcaagaa gaaggacaag 241 gaactgaagt tgttcaagga gtgcttttgg gtctggttgt agaagatcgg cttgaaatta 301 ccaactgctt tcctttccct cagcacacag aggatgatgc tgactttgat gaagtccaat 361 atcagatgga aatgatgcgg agccttcgcc atgtaaacat tgatcatctt cacgtgggct 421 ggtatcagtc cacatactat ggctcattcg ttacccgggc actcctggac tctcagttta 481 gttaccagca tgccattgaa gaatctgtcg ttctcattta tgatcccata aaaactgccc 541 aaggatctct ctcactaaag gcatacagac tgactcctaa actgatggaa gtttgtaaag 601 aaaaggattt ttcccctgaa gcattgaaaa aagcaaatat cacctttgag tacatgtttg 661 aagaagtgcc gattgtaatt aaaaattcac atctgatcaa tgtcctaatg tgggaacttg 721 aaaagaagtc agctgttGCA GATAAACATG AATTGCTCAG CCTTGCCAGC AGCAATCATT 781 TGGGGAAGAA TCTACAGTTG CTGATGGACA GAGTGGATGA AATGAGCCAA GATATAGTTA 841 AATACAACAC ATACATGAGG AATACTAGTA AACAACAGCA GCAGAAACAT CAGTATCAGC 901 AGCGTCGCCA GCAGGAGAAT ATGCAGCGCC AGAGCCGAGG AGAACCCCCG CTCCCTGAGG 961 AGGACCTGTC CAAACTCTTC AAACCACCAC AGCCGCCTGC CAGGATGGAC TCGCTGCTCA 1021 TTGCAGGCCA GATAAACACT TACTGCCAGA ACATCAAGGA GTTCACTGCC CAAAACTTAG 1081 GCAAGCTCTT CATGGCCCAG GCTCTTCAAG AATACAACAA CTACCCAACT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGATAT AGGTCTAACG CGAATTGTGG GACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t