Construct: ORF TRCN0000491417
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020902.2_s317c1
- DNA Barcode:
- AGGAAAAACAATTCAGCATAGCAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADGRL4 (64123)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491417
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64123 | ADGRL4 | adhesion G protein-coupled ... | NM_022159.4 | 87.7% | 87.6% | 1_252del;898G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1890
- ORF length:
- 1818
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtgtgta cctggcttca gatccagcag taaccaagac aggtttatca 121 ctaatgatgg aaccgtctgt atagaaaatg tgaatgcaaa ctgccattta gataatgtct 181 gtatagctgc aaatattaat aaaactttaa caaaaatcag atccataaaa gaacctgtgg 241 ctttgctaca agaagtctat agaaattctg tgacagatct ttcaccaaca gatataatta 301 catatataga aatattagct gaatcatctt cattactagg ttacaagaac aacactatct 361 cagccaagga caccctttct aactcaactc ttactgaatt tgtaaaaacc gtgaataatt 421 ttgttcaaag ggatacattt gtagtttggg acaagttatc tgtgaatcat aggagaacac 481 atcttacaaa actcatgcac actgttgaac aagctacttt aaggatatcc cagagcttcc 541 aaaagaccac agagtttgat acaaattcaa cggatatagc tctcaaagtt ttcttttttg 601 attcatataa catgaaacat attcatcctc atatgaatat ggatggagac tacataaata 661 tatttccaaa gagaaaagct gcatatgatt caaatggcaa tgttgcagtt gcatttttat 721 attataagag tattggtcct ttgctttcat catctgacaa cttcttattg aaacctcaaa 781 attatgataa ttctgaagag gaggaaagag tcatatcttc agtaatttca gtctcaatga 841 gctcaaaccc acccacatta tatgaacttg aaaaaataac atttacatta agtcatcgaa 901 aggtcacaga taggtatagg agtctatgtg cattttggaa ttactcacct gataccatga 961 atggcagctg gtcttcagag ggctgtgagc tgacatactc aaatgagacc cacacctcat 1021 gccgctgtaa tcacctgaca cattttgcaa ttttgatgtc ctctggtcct tccattggta 1081 ttaaagatta taatattctt acaaggatca ctcaactagg aataattatt tcactgattt 1141 gtcttgccat atgcattttt accttctggt tcttcagtga aattcaaagc accaggacaa 1201 caattcacaa aaatctttgc tgtagcctat ttcttgctga acttgttttt cttgttggga 1261 tcaatacaaa tactaataag ctcttctgtt caatcattgc cggactgcta cactacttct 1321 ttttagctgc ttttgcatgg atgtgcattg aaggcataca tctctatctc attgttgtgg 1381 gtgtcatcta caacaaggga tttttgcaca agaattttta tatctttggc tatctaagcc 1441 cagccgtggt agttggattt tcggcagcac taggatacag atattatggc acaaccaaag 1501 tatgttggct tagcaccgaa aacaacttta tttggagttt tataggacca gcatgcctaa 1561 tcattcttgt taatctcttg gcttttGGAG TCATCATATA CAAAGTTTTT CGTCACACTG 1621 CAGGGTTGAA ACCAGAAGTT AGTTGCTTTG AGAACATAAG GTCTTGTGCA AGAGGAGCCC 1681 TCGCTCTTCT GTTCCTTCTC GGCACCACCT GGATCTTTGG GGTTCTCCAT GTTGTGCACG 1741 CATCAGTGGT TACAGCTTAC CTCTTCACAG TCAGCAATGC TTTCCAGGGG ATGTTCATTT 1801 TTTTATTCCT GTGTGTTTTA TCTAGAAAGA TTCAAGAAGA ATATTACAGA TTGTTCAAAA 1861 ATGTCCCCTG TTGTTTTGGA TGTTTAAGGT AGGACCCAGC TTTCTTGTAC AAAGTGGTTG 1921 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1981 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 2041 GAAAAACAAT TCAGCATAGC ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 2101 ttgtgaaaga tt