Construct: ORF TRCN0000491433
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019724.2_s317c1
- DNA Barcode:
- GCACACCCCTGGTCATCGCATCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IRAK3 (11213)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491433
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11213 | IRAK3 | interleukin 1 receptor asso... | NM_007199.3 | 99.8% | 99.6% | 439A>G;1788_1789insG |
| 2 | human | 11213 | IRAK3 | interleukin 1 receptor asso... | NM_001142523.2 | 89.6% | 89.4% | 132_133ins183;256A>G;1605_1606insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1860
- ORF length:
- 1791
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcggggaac tgtggggccc gcggcgcgct gtcggcgcac acgctgctgt 121 tcgacctgcc gcccgcgctg ctcggagagc tctgcgctgt tctggacagc tgcgacggcg 181 cgctgggctg gcgcggcctg gcagagagac tttcaagcag ctggctggat gttcgtcata 241 ttgaaaagta tgtagaccaa ggtaaaagtg gaacaagaga attactttgg tcctgggcac 301 agaaaaacaa gaccatcggt gaccttttac aggtcctcca ggagatggga catcgtcgag 361 ctattcattt aattacaaac tatggagcag tgttgagtcc ttcagagaag agttatcagg 421 aaggtggatt tccaaatata ttattcaagg aaacagccaa tgtcaccgtg gataatgttc 481 ttattcctga acataatgaa aaaggagtac tgcttaaatc ttccatcagc tttcaaaata 541 tcatagaagg aactagaaat ttccacaaag acttcctaat tggagaagga gagatttttg 601 aggtatacag agtggagatt caaaacctaa catatgctgt caaattattt aaacaggaga 661 aaaaaatgca gtgtaagaag cattggaaga ggtttttatc tgagcttgaa gttttactac 721 tgtttcatca cccaaacata ctagagttgg ctgcatattt tacagagact gagaagttct 781 gtctgattta tccatacatg agaaatggaa cactttttga cagattgcag tgtgtaggtg 841 acacggcccc actcccttgg cacattcgaa tcggtatatt aataggaata tccaaagcca 901 ttcactacct gcacaacgtt caaccatgct cggtcatctg tggcagtata tcaagtgcaa 961 acatcctttt ggatgatcag tttcaaccca aactaactga ttttgccatg gcacacttcc 1021 ggtcccacct agaacatcag agttgtacca taaatatgac cagcagcagc agtaaacatc 1081 tgtggtacat gccagaagag tacatcagac aggggaaact ttccattaaa acagatgtct 1141 acagctttgg aattgtaata atggaagttc taacaggatg tagagtagtg ttagatgatc 1201 caaaacatat ccagctgcgg gatctcctta gagaattgat ggagaagaga ggcctggatt 1261 catgtctctc atttctagat aagaaagtgc ctccctgccc tcggaatttc tctgccaagc 1321 tcttctgttt ggcaggccgg tgtgctgcaa cgcgggcaaa gttaagacca tcaatggatg 1381 aagttttaaa tactcttgaa agtactcaag ccagcttgta ttttgctgaa gatcctccca 1441 catcactaaa gtccttcagg tgtccttctc ctctattcct ggagaatgta ccaagtattc 1501 cagtggaaga tgatgaaagc cagaataaca atttactacc ttctgatgaa ggccTGAGGA 1561 TAGACAGAAT GACTCAGAAA ACTCCTTTTG AATGCAGCCA GTCTGAGGTT ATGTTTCTGA 1621 GCTTGGACAA AAAGCCAGAG AGCAAGAGAA ATGAGGAAGC TTGCAACATG CCCAGTTCTT 1681 CTTGTGAAGA AAGTTGGTTC CCAAAGTATA TAGTTCCATC CCAGGACTTA AGGCCCTATA 1741 AGGTAAATAT AGATCCTTCT TCAGAAGCTC CAGGGCATTC TTGCAGGAGC AGGCCAGTGG 1801 AGAGCAGCTG TTCCTCCAAA TTTTCCTGGG ATGAATATGA ACAGTACAAA AAAGAAGACC 1861 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1921 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1981 ATATATCTTG TGGAAAGGAC GAGCACACCC CTGGTCATCG CATCAAACGC GTTAAGTCga 2041 caatcaacct ctggattaca aaatttgtga aagatt