Construct: ORF TRCN0000491443
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019513.2_s317c1
- DNA Barcode:
- ATGACACTGGGACTCTAGCTCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TESK2 (10420)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491443
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10420 | TESK2 | testis associated actin rem... | NM_007170.3 | 46.6% | 46.5% | 1_141del;143_144delGTinsTG;943_1713del |
| 2 | human | 10420 | TESK2 | testis associated actin rem... | NM_001320800.2 | 44% | 44% | 0_1ins108;694_1464del |
| 3 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | NM_146151.4 | 42.3% | 44.2% | (many diffs) |
| 4 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | XM_006503008.3 | 34.3% | 35.9% | (many diffs) |
| 5 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | XR_001784124.1 | 21.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 87
- ORF end:
- 888
- ORF length:
- 801
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcacctct agaactatgg ccttttccag actgacgcgt ttggatgatt 121 tcacctgtga aaaaataggg tctggcttct tttctgaagt gttcaaggta cgacaccgag 181 cttctggtca ggtgatggct cttaagatga acacattgag cagtaaccgg gcaaacatgc 241 tgaaagaagt acagctcatg aatagactct cccatcccaa catccttagg ttcatgggtg 301 tatgtgttca tcaaggacaa ttgcatgcac ttacagagta tatcaactcc gggaacctgg 361 aacagttgct agacagtaac ctgcatttgc cttggactgt gagggtaaaa ctggcctatg 421 acatagcagt gggcctcagc taccttcact tcaaaggcat ttttcatcgg gacctcacat 481 ctaagaactg cctgataaag agggatgaga atggttactc tgcagtggta gctgactttg 541 gcctggctga gaagatcccc gatgtcagca tggggagtga gaagctggcc gtggtgggtt 601 ccccattctg gatggcacct gaggttcTCC GAGATGAGCC CTATAATGAA AAGGCAGATG 661 TGTTCTCTTA TGGTATCATC CTCTGCGAGA TCATCGCCCG CATCCAGGCC GATCCGGACT 721 ATCTTCCCCG CACAGAGAAT TTCGGGCTGG ACTATGATGC TTTCCAGCAC ATGGTGGGAG 781 ACTGTCCCCC AGATTTTCTG CAACTTACTT TCAACTGCTG TAACATGGAT CCCAAACTGC 841 GCCCATCTTT TGTGGAGATT GGGAAGACCC TGGAGGAAAT TCTGAGCAAG GGTGGGCGCG 901 CCGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 961 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1021 GGCTTTATAT ATCTTGTGGA AAGGACGAAT GACACTGGGA CTCTAGCTCT TTACGCGTTA 1081 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt