Construct: ORF TRCN0000491510
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021809.2_s317c1
- DNA Barcode:
- CGAAAACTGTTATGCAAGCGATCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BTN2A1 (11120)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491510
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_078476.4 | 100% | 100% | |
| 2 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_001197234.3 | 98.6% | 97.9% | (many diffs) |
| 3 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_001197238.2 | 78% | 72.3% | (many diffs) |
| 4 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_006714955.3 | 76.1% | 70.3% | (many diffs) |
| 5 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010168.2 | 76.1% | 70.3% | (many diffs) |
| 6 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010169.1 | 76.1% | 70.3% | (many diffs) |
| 7 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_007049.5 | 63.1% | 62.5% | (many diffs) |
| 8 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_001197240.2 | 58.6% | 51.5% | (many diffs) |
| 9 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_001197237.2 | 51.9% | 47.9% | (many diffs) |
| 10 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_006995.5 | 51.9% | 47.9% | (many diffs) |
| 11 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_005248797.3 | 51.9% | 47.9% | (many diffs) |
| 12 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_006714953.4 | 51.9% | 47.9% | (many diffs) |
| 13 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_011514228.2 | 51.9% | 47.9% | (many diffs) |
| 14 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_001197233.2 | 51.5% | 50.9% | (many diffs) |
| 15 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_011514229.2 | 42% | 38.9% | (many diffs) |
| 16 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010166.2 | 42% | 38.9% | (many diffs) |
| 17 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_024446302.1 | 42% | 38.9% | (many diffs) |
| 18 | human | 54718 | BTN2A3P | butyrophilin subfamily 2 me... | NR_027795.1 | 36.4% | (many diffs) | |
| 19 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_181531.3 | 31.9% | 28.2% | (many diffs) |
| 20 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_005248798.4 | 31.9% | 28.2% | (many diffs) |
| 21 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010167.1 | 31.9% | 28.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1071
- ORF length:
- 1002
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggaatcagct gctgccctgc acttctcccg gccagcctcc ctcctcctcc 121 tcctcctcag cctgtgtgca ctggtctcag cccagtttat tgtcgtgggg cccactgatc 181 ccatcttggc cacggttgga gaaaacacta cgttacgctg ccatctgtca cccgagaaaa 241 atgctgagga catggaggtg cggtggttcc ggtctcagtt ctcccccgca gtgtttgtgt 301 ataaaggtgg cagagagaga acagaggagc agatggagga gtaccgagga agaaccacct 361 ttgtgagcaa agacatcagc aggggcagcg tggccctggt catacacaac atcacagccc 421 aggaaaacgg cacctaccgc tgttacttcc aagaaggcag gtcctacgat gaggccatcc 481 tgcacctcgt agtggcagga ctaggctcta agcccctcat ttcaatgagg ggccatgaag 541 acgggggcat ccggctggag tgcatatcta gagggtggta cccaaagccc ctcacagtgt 601 ggagggaccc ctacggtggg gttgcgcctg ccctgaaaga ggtctccatg cctgatgcag 661 acggcctctt catggtcacc acggctgtga tcatcagaga caagtctgtg aggaacatgt 721 cctgctctat caacaacacc ctgctcggcc agaagaaaga aagtgtcatt tttattccag 781 aaTCCTTTAT GCCCAGTGTG TCTCCCTGTG CAGTGGCCCT GCCTATCATT GTGGTTATTC 841 TGATGATACC CATTGCCGTA TGCATCTATT GGATCAACAA ACTCCAAAAG GAAAAAAAGA 901 TTCTGTCAGG GGAAAAGGAG TTTGAACGGG AAACAAGAGA AATTGCTCTA AAGGAACTGG 961 AGAAAGAACG TGTGCAAAAA GAGGAAGAAC TTCAAGTAAA AGAGAAACTT CAAGAAGAAT 1021 TGCGATGGAG AAGAACATTC TTACATGCTG AGCTCCAATT CTTCTCAAAC TAGAACCCAG 1081 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1141 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1201 TATCTTGTGG AAAGGACGAC GAAAACTGTT ATGCAAGCGA TCTACGCGTT AAGTCgacaa 1261 tcaacctctg gattacaaaa tttgtgaaag att