Construct: ORF TRCN0000491551
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019693.2_s317c1
- DNA Barcode:
- GCATCTATTGGCCGTACTCTGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDXK (8566)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491551
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8566 | PDXK | pyridoxal kinase | NM_003681.5 | 99.8% | 99.6% | 936_937insG |
| 2 | human | 8566 | PDXK | pyridoxal kinase | XM_005261196.3 | 90.7% | 86.1% | (many diffs) |
| 3 | human | 8566 | PDXK | pyridoxal kinase | NM_001331030.2 | 86.8% | 84.9% | (many diffs) |
| 4 | human | 8566 | PDXK | pyridoxal kinase | XM_011529758.3 | 81.7% | 73.5% | (many diffs) |
| 5 | human | 8566 | PDXK | pyridoxal kinase | XM_005261199.2 | 76.5% | 76.3% | 0_1ins219;717_718insG |
| 6 | human | 8566 | PDXK | pyridoxal kinase | XM_011529760.2 | 76.5% | 76.3% | 0_1ins219;717_718insG |
| 7 | human | 8566 | PDXK | pyridoxal kinase | XM_011529761.1 | 76.5% | 76.3% | 0_1ins219;717_718insG |
| 8 | human | 8566 | PDXK | pyridoxal kinase | XM_017028483.2 | 76.5% | 76.3% | 0_1ins219;717_718insG |
| 9 | human | 8566 | PDXK | pyridoxal kinase | XM_017028484.1 | 76.5% | 76.3% | 0_1ins219;717_718insG |
| 10 | human | 8566 | PDXK | pyridoxal kinase | XM_005261195.2 | 74.8% | 70.5% | (many diffs) |
| 11 | mouse | 216134 | Pdxk | pyridoxal (pyridoxine, vita... | NM_172134.2 | 84.7% | 85.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1014
- ORF length:
- 939
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag gaggagtgcc gggtgctctc catacagagc cacgtcatcc 121 gcggctacgt gggcaaccgg gcggccacgt tcccgctgca ggttttggga tttgagattg 181 acgcggtgaa ctctgtccag ttttcaaacc acacaggcta tgcccactgg aagggccaag 241 tgctgaattc agatgagctc caggagttgt acgaaggcct gaggctgaac aacatgaata 301 aatatgacta cgtgctcaca ggttatacga gggacaagtc gttcctggcc atggtggtgg 361 acattgtgca ggagctgaag cagcagaacc ccaggctggt gtacgtgtgt gatccagtct 421 tgggtgacaa gtgggacggc gaaggctcga tgtacgtccc ggaggacctc cttcccgtct 481 acaaagaaaa agtggtgccg cttgcagaca ttatcacgcc caaccagttt gaggccgagt 541 tactgagtgg ccggaagatc cacagccagg aggaagcctt gcgggtgatg gacatgctgc 601 actctatggg ccccgacacc gtggtcatca ccagctccga cctgccctcc ccgcagggca 661 gcaactacct gattgtgctg gggagtcaga ggaggaggaa TCCCGCTGGC TCCGTGGTGA 721 TGGAACGCAT CCGGATGGAC ATTCGCAAAG TGGACGCCGT CTTTGTGGGC ACTGGGGACC 781 TGTTTGCTGC CATGCTCCTG GCGTGGACAC ACAAGCACCC CAATAACCTC AAGGTGGCCT 841 GTGAGAAGAC CGTGTCTACC TTGCACCACG TTCTGCAGAG GACCATCCAG TGTGCAAAAG 901 CCCAGGCCGG GGAAGGAGTG AGGCCCAGCC CCATGCAGCT GGAGCTGCGG ATGGTGCAGA 961 GCAAAAGGGA CATCGAGGAC CCAGAGATCG TCGTCCAGGC CACGGTGCTG GACCCAGCTT 1021 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1081 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1141 CTTGTGGAAA GGACGAGCAT CTATTGGCCG TACTCTGGCG ACGCGTTAAG TCgacaatca 1201 acctctggat tacaaaattt gtgaaagatt