Construct: ORF TRCN0000491555
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005599.1_s317c1
- Derived from:
- ccsbBroadEn_13759
- DNA Barcode:
- TAATCTGGTCAAATATTAATTCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491555
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001178126.2 | 64.4% | 56% | (many diffs) |
| 2 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001256296.2 | 54.5% | 49.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 774
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctggtctcct ctcctcctca ctctcctcgc tcactgcaca gggtcctggg 121 cccagtctgt tctggcgcag ccgccctcag tgtctggggc cccagggcag acggtcacca 181 tctcgtgcac tgggagcagc accaacatcg gggcgggtta tgctgtacac tggtaccagc 241 agtttccagg agcggccccc aaagtcctca tctatggtaa ctacaatcgg ccctcagggg 301 tccctgaccg attctctggc tccaagtctg gcacctcagc ctccctggcc atcactgggc 361 tccaggctga agatgaggct gattattact gccagtcgta tgacggcagc ctgagtggtt 421 ctgtcttcgg agctgggacc aaggtcaccg tcctgggtca gcccaaggcc aaccccactg 481 tcactctgtt cccgcccTCC TCTGAGGAGC TCCAAGCCAA CAAGGCCACA CTAGTGTGTC 541 TGATCAGTGA CTTCTACCCG GGAGCTGTGA CAGTGGCCTG GAAGGCAGAT GGCAGCCCCG 601 TCAAGGCGGG AGTGGAGACC ACCAAACCCT CCAAACAGAG CAACAACAAG TACGCGGCCA 661 GCAGCTACCT GAGCCTGACG CCCGAGCAGT GGAAGTCCCA CAGAAGCTAC AGCTGCCAGG 721 TCACGCATGA AGGGAGCACC GTGGAGAAGA CAGTGGCCCC TACAGAATGT TCATACCCAA 781 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 841 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 901 TATCTTGTGG AAAGGACGAT AATCTGGTCA AATATTAATT CACACGCGTT AAGTCgacaa 961 tcaacctctg gattacaaaa tttgtgaaag att