Construct: ORF TRCN0000491565
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020315.2_s317c1
- DNA Barcode:
- GAAGACTCTAAACACCATTAATAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NIM1K (167359)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491565
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 167359 | NIM1K | NIM1 serine/threonine prote... | NM_153361.4 | 99.9% | 100% | 45C>T |
| 2 | human | 167359 | NIM1K | NIM1 serine/threonine prote... | NR_072980.1 | 44.3% | 1_1499del;1544C>T;2808_2944del | |
| 3 | mouse | 245269 | Nim1k | NIM1 serine/threonine prote... | NM_175538.3 | 87.3% | 91.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1380
- ORF length:
- 1308
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgactgca gtgtatatga atggaggtgg cctggtgaac ccccattatg 121 cccggtggga tcggcgcgac agtgtagaaa gtggctgtca gaccgagagt agcaaggagg 181 gtgaggaggg acagccccgc cagctgacgc ccttcgagaa actgacacag gacatgtccc 241 aggatgagaa ggtggtgagg gagatcacgc tggggaaacg gataggcttc taccgaattc 301 gaggggaaat cggaagtgga aacttctccc aagtgaagct tgggattcac tccctaacca 361 aagaaaaggt ggccattaag atcctggaca agaccaagtt agaccagaaa acccagaggc 421 tactatcccg agaaatctcc agcatggaaa agctgcacca tcccaacatc atccgccttt 481 acgaagtggt ggagacccta tccaagctgc acttggtgat ggagtatgca gggggtgggg 541 agctcttcgg aaaaattagc actgagggga agctctctga accagaaagc aagctcatct 601 tctcccagat tgtgtctgcc gtgaagcaca tgcatgaaaa ccaaattatt catagagatc 661 tgaaagcaga aaatgtattc tataccagta atacttgtgt gaaggtgggc gattttggat 721 tcagcacagt aagcaaaaaa ggtgaaatgc tgaacacttt ctgtgggtct cctccctacg 781 ctgcgcctga actcttccgg gacgagcact acatcggcat ttacgtggat atctgggcct 841 tgggggtgct tttgtacttc atggtgactg gcaccatgcc atttcgggca gaaaccgtgg 901 ccaaactaaa aaagagcatc ctcgagggca catacagtgt accgccgcac gtgtcagagc 961 cctgccaccg actcatccga ggagtccttc agcagatccc cacggagagg tacggaatcg 1021 actgcatcat gaatgatgaa tGGATGCAAG GGGTGCCATA CCCTACACCT TTGGAACCTT 1081 TCCAACTGGA TCCCAAACAT TTGTCGGAAA CCAGCACTCT CAAGGAAGAA GAAAATGAGG 1141 TCAAAAGCAC TTTAGAACAT TTGGGCATTA CAGAAGAGCA TATTCGAAAT AACCAAGGGA 1201 GAGATGCTCG CAGCTCAATC ACAGGGGTCT ATAGAATTAT TTTACATAGA GTCCAAAGGA 1261 AGAAGGCTTT GGAAAGTGTC CCAGTCATGA TGCTACCAGA CCCTAAAGAA AGAGACCTCA 1321 AAAAAGGGTC CCGTGTCTAC AGAGGGATAA GACACACATC CAAATTTTGC TCGATTTTAT 1381 GAGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1441 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1501 GGCTTTATAT ATCTTGTGGA AAGGACGAGA AGACTCTAAA CACCATTAAT ACACGCGTTA 1561 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt