Construct: ORF TRCN0000491581
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003879.2_s317c1
- Derived from:
- ccsbBroadEn_09867
- DNA Barcode:
- TCAGTCCAATGTGAACCAATGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SKA3 (221150)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491581
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221150 | SKA3 | spindle and kinetochore ass... | NM_145061.6 | 99.8% | 99.7% | 172G>A;1086G>A |
| 2 | human | 221150 | SKA3 | spindle and kinetochore ass... | NM_001166017.2 | 92.9% | 88.8% | (many diffs) |
| 3 | human | 221150 | SKA3 | spindle and kinetochore ass... | XM_011534994.2 | 90.1% | 90% | 0_1ins120;52G>A;966G>A |
| 4 | human | 221150 | SKA3 | spindle and kinetochore ass... | XM_005266288.4 | 80% | 80% | 0_1ins246;840G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1302
- ORF length:
- 1236
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ccctatccgg agcttctgcg ggaagctgcg gtctctggcc agcacgctgg 121 actgcgagac ggcccggctg cagcgagcgc tggacggaga ggaaagcgac tttgaagatt 181 atccaatgag aattttatat gaccttcatt cagaagttca gactctaaag gatgatatta 241 atattcttct tgataaagca agattggaaa atcaagaagg cattgatttc ataaaggcaa 301 caaaagtact aatggaaaaa aattcaatgg atattatgaa aataagagag tatttccaga 361 agtatggata tagtccacgt gtcaagaaaa attcagtaca cgagcaagaa gccattaact 421 ctgacccaga gttgtctaat tgtgaaaatt ttcagaagac tgatgtgaaa gatgatctgt 481 ctgatcctcc tgttgcaagc agttgtattt ctgagaagtc tccacgtagt ccacaacttt 541 cagattttgg acttgagcgg tacatcgtat cccaagttct accaaaccct ccacaggcag 601 tgaacaacta taaggaagag cccgtaattg taaccccacc taccaaacaa tcactagtaa 661 aagtactaaa aactccaaaa tgtgcactaa aaatggatga ttttgagtgt gtaactccta 721 aattagaaca ctttggtatc tctgaatata ctatgtgttt aaatgaagat tacacaatgg 781 gacttaaaaa tgcgaggaat aataaaagtg aggaggccat agatacagaa tccaggctca 841 atgataatgt ttttgccact cccagcccca tcatccagca gttggaaaaa agtgatgccg 901 aatataccaa ctctcctttg gtacctaCAT TCTGTACTCC TGGTTTGAAA ATTCCATCTA 961 CAAAGAACAG CATAGCTTTG GTATCCACAA ATTACCCATT ATCAAAAACA AATAGTTCAT 1021 CAAATGATTT GGAAGTTGAA GATCGTACTT CGTTGGTTTT AAATTCAGAC ACATGCTTTG 1081 AGAATTTAAC AGATCCCTCT TCACCTACGA TTTCTTCTTA TGAGAATCTG CTCAGAACAC 1141 CTACACCTCC AGAAGTAACT AAAATTCCAG AAGATATTCT CCAGCTTTTA TCAAAATACA 1201 ACTCAAACCT AGCTACTCCA ATAGCAATTA AAGCAGTGCC ACCCAGTAAA AGGTTCCTTA 1261 AACATGGACA GAACATCCGA GATGTCAGCA ACAAAGAAAA CTGCCCAACT TTCTTGTACA 1321 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1381 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1441 AGGACGATCA GTCCAATGTG AACCAATGGT TACGCGTTAA GTCgacaatc aacctctgga 1501 ttacaaaatt tgtgaaagat t