Construct: ORF TRCN0000491612
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021053.2_s317c1
- DNA Barcode:
- AGCACGTATGATGGACTAACAGGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GPR39 (2863)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491612
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2863 | GPR39 | G protein-coupled receptor 39 | NM_001508.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1431
- ORF length:
- 1359
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcttca cccagcctcc cgggcagtga ctgctcccaa atcattgatc 121 acagtcatgt ccccgagttt gaggtggcca cctggatcaa aatcaccctt attctggtgt 181 acctgatcat cttcgtgatg ggccttctgg ggaacagcgc caccattcgg gtcacccagg 241 tgctgcagaa gaaaggatac ttgcagaagg aggtgacaga ccacatggtg agtttggctt 301 gctcggacat cttggtgttc ctcatcggca tgcccatgga gttctacagc atcatctgga 361 atcccctgac cacgtccagc tacaccctgt cctgcaagct gcacactttc ctcttcgagg 421 cctgcagcta cgctacgctg ctgcacgtgc tgacactcag ctttgagcgc tacatcgcca 481 tctgtcaccc cttcaggtac aaggctgtgt cgggaccttg ccaggtgaag ctgctgattg 541 gcttcgtctg ggtcacctcc gccctggtgg cactgccctt gctgtttgcc atgggtactg 601 agtaccccct ggtgaacgtg cccagccacc ggggtctcac ttgcaaccgc tccagcaccc 661 gccaccacga gcagcccgag acctccaata tgtccatctg taccaacctc tccagccgct 721 ggaccgtgtt ccagtccagc atcttcggcg ccttcgtggt ctacctcgtg gtcctgctct 781 ccgtagcctt catgtgctgg aacatgatgc aggtgctcat gaaaagccag aagggctcgc 841 tggccggggg cacgcggcct ccgcagctga ggaagtccga gagcgaagag agcaggaccg 901 ccaggaggca gaccatcatc ttcctgaggc tgattgttgt gacattggcc gtatgctgga 961 tgcccaacca gattcggagg atcatggctg cggccaaacc caagcacgac tggacgaggt 1021 cctacttccg ggcgtacatg atcctcctcc ccttctcgga gacgtttttc tacctcagct 1081 cggtcatcaa cccgcTCCTG TACACGGTGT CCTCGCAGCA GTTTCGGCGG GTGTTCGTGC 1141 AGGTGCTGTG CTGCCGCCTG TCGCTGCAGC ACGCCAACCA CGAGAAGCGC CTGCGCGTAC 1201 ATGCGCACTC CACCACCGAC AGCGCCCGCT TTGTGCAGCG CCCGTTGCTC TTCGCGTCCC 1261 GGCGCCAGTC CTCTGCAAGG AGAACTGAGA AGATTTTCTT AAGCACTTTT CAGAGCGAGG 1321 CCGAGCCCCA GTCTAAGTCC CAGTCATTGA GTCTCGAGTC ACTAGAGCCC AACTCAGGCG 1381 CGAAACCAGC CAATTCTGCT GCAGAGAATG GTTTTCAGGA GCATGAAGTT TGAGACCCAG 1441 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1501 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1561 TATCTTGTGG AAAGGACGAA GCACGTATGA TGGACTAACA GGAACGCGTT AAGTCgacaa 1621 tcaacctctg gattacaaaa tttgtgaaag att